Department of Pharmacology & Toxicology, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia.
Environ Pollut. 2013 Oct;181:226-32. doi: 10.1016/j.envpol.2013.06.014. Epub 2013 Jul 17.
The present study was designed to evaluate the influence of long-term environmental human exposure to three heavy metals, lead (Pb), cadmium (Cd), and mercury (Hg), on the expression of detoxifying, xenobiotic metabolizing, and DNA repair genes in Mahd Ad-Dahab city. The study groups consisted of 40 healthy male residents (heavy metal-exposed) and 20 healthy male from Riyadh city, 700 km away, and served as control group. The heavy metal-exposed group with high exposure to Pb, Cd, or Hg was divided into three subgroups Pb-, Cd-, and Hg-exposed groups, respectively. The mRNA expression levels of detoxifying, NQO1, HO-1, GSTA1, MT-1, and HSP70, were significantly decreased in all heavy metal-exposed group as compared to control group. This was accompanied with a proportional decrease in the expression of xenobiotic metabolizing gene, cytochrome P4501A1. On the other hand, the DNA repair gene OGG1 and the 8-OHdG level were dramatically inhibited in Cd-exposed group only.
本研究旨在评估长期环境中人类暴露于三种重金属（铅、镉和汞）对 Mahd Ad-Dahab 市解毒、外源性代谢物和 DNA 修复基因表达的影响。研究组由 40 名来自 Mahd Ad-Dahab 市（重金属暴露组）的健康男性居民和 20 名来自 700 公里外的利雅得市的健康男性组成，作为对照组。高暴露于 Pb、Cd 或 Hg 的重金属暴露组分别分为 Pb、Cd 和 Hg 暴露组。与对照组相比，所有重金属暴露组的解毒、NQO1、HO-1、GSTA1、MT-1 和 HSP70 的 mRNA 表达水平均显著降低。同时，外源性代谢物基因细胞色素 P4501A1 的表达也呈比例下降。另一方面，仅在 Cd 暴露组中，DNA 修复基因 OGG1 和 8-OHdG 水平受到显著抑制。
