Drug Applied Research Center, Tabriz University of Medical Sciences, P.O. Box 51664, Tabriz, Iran.
J Neurosci Methods. 2013 Sep 30;219(1):52-60. doi: 10.1016/j.jneumeth.2013.07.007. Epub 2013 Jul 16.
Simultaneous measurement of dopamine and serotonin levels is of greater value because of their coexistence in biological systems. These facts have encouraged chemists to develop faster, simpler, and more sensitive techniques to meet the various demands and many works have been published describing the measurement of serum dopamine and serotonin concentrations using chemically modified electrodes.
Electrochemical techniques based on various chemically modified electrodes have been widely explored for the single determination of dopamine and serotonin in vivo or in vitro. To the best of our knowledge, there are only limit reports on the electrochemical determination of dopamine and serotonin using mesoporous materials. On the other hand, simultaneous measurement of dopamine and serotonin concentrations on the basis of silica based mesoporous materials has not been reported.
In the present work, the development an effective electroanalytical method for the simultaneous determination of dopamine and serotonin using cyclic and differential pulse voltammetry on the mobile crystalline material-41 functionalized by n-aminopropyl is described. Mobile crystalline material-41 functionalized by n-aminopropyl, a kind of mesoporous silica, was synthesized and then used to prepare an electrochemical device for simultaneous determination of dopamine and serotonin. A validated differential pulse voltammetry procedure was described to assay two neurotransmitters separately or combined in human serum.
The prepared sensor shows voltammetric responses with high sensitivity for dopamine and serotonin in optimal conditions, which makes it very suitable for simultaneous determination of these compounds.
由于多巴胺和血清素在生物系统中共存，因此同时测量它们的水平更有价值。这些事实促使化学家开发更快、更简单、更灵敏的技术来满足各种需求，许多工作已经发表，描述了使用化学修饰电极测量血清多巴胺和血清素浓度。
基于各种化学修饰电极的电化学技术已广泛用于体内或体外多巴胺和血清素的单一测定。据我们所知，只有有限的报道涉及使用介孔材料电化学测定多巴胺和血清素。另一方面，基于基于二氧化硅的介孔材料同时测量多巴胺和血清素浓度尚未报道。
在本工作中，开发了一种使用循环伏安法和差分脉冲伏安法在由 n-氨丙基功能化的移动结晶材料-41 上同时测定多巴胺和血清素的有效电分析方法。移动结晶材料-41 由 n-氨丙基功能化，是一种介孔硅，被合成并用于制备用于同时测定多巴胺和血清素的电化学装置。描述了一种经过验证的差分脉冲伏安法程序，用于分别或组合测定人血清中的两种神经递质。
在最佳条件下，制备的传感器对多巴胺和血清素具有高灵敏度的伏安响应，非常适合同时测定这些化合物。
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