College of Chemistry & Chemical Engineering, Lanzhou University, Lanzhou 730000, PR China; College of Chemistry & Chemical Engineering, Qinghai Normal University, Xining 810000, PR China.
College of Chemistry & Chemical Engineering, Lanzhou University, Lanzhou 730000, PR China.
Bioelectrochemistry. 2021 Jun;139:107739. doi: 10.1016/j.bioelechem.2021.107739. Epub 2021 Jan 11.
An electrochemical method combining chemometrics was developed for simultaneous quantification of multiple neurotransmitters including Dopamine (DA), Epinephrine (EP), Norepinephrine (NE) and serotonin (5-hydroxytryptamine, 5-HT) in human blood serum. A reduced graphene oxide modified glassy carbon electrode (RGO/GCE) was prepared via electrodeposition method. Differential pulse voltammetry (DPV) measurement of the four neurotransmitters showed that the voltammetric signals of the four targets overlapped significantly. To facilitate the simultaneous determination of the neurotransmitters, a chemometric tool of Tchebichef curve moment (TcM) method was proposed. The TcMs calculated from the voltammograms were used to establish the quantitative models by stepwise regression. The intra-day and inter-day precisions of the proposed method were less than 3.5% and 8.1%, respectively, and the recoveries were from 87.4% to 124%. The limit of detection (LOD) for DA, EP, NE and 5-HT were 74 nM, 104 nM, 84 nM and 97 nM, respectively. The above results indicated that the proposed approach is simple and reliable for the simultaneous determination of multiple neurotransmitters in human serum.
电化学方法结合化学计量学被开发用于同时定量分析人血清中的多种神经递质，包括多巴胺（DA）、肾上腺素（EP）、去甲肾上腺素（NE）和 5-羟色胺（5-HT）。通过电沉积法制备了还原氧化石墨烯修饰的玻碳电极（RGO/GCE）。对四种神经递质的差分脉冲伏安法（DPV）测量表明，四个目标的伏安信号重叠非常明显。为了便于同时测定神经递质，提出了一种 Tchebichef 曲线矩（TcM）方法的化学计量学工具。从伏安图中计算出的 TcM 用于通过逐步回归建立定量模型。该方法的日内和日间精密度均小于 3.5%和 8.1%，回收率为 87.4%至 124%。DA、EP、NE 和 5-HT 的检测限（LOD）分别为 74 nM、104 nM、84 nM 和 97 nM。结果表明，该方法简单可靠，可用于同时测定人血清中的多种神经递质。
