布雷菲德菌素 A 抑制莱茵衣藻三酰甘油的动员。
Triacylglycerol mobilization is suppressed by brefeldin A in Chlamydomonas reinhardtii.
机构信息
Department of Biological Sciences, Louisiana State University, 226 Life Sciences Building, Baton Rouge, LA 70803, USA.
出版信息
Plant Cell Physiol. 2013 Oct;54(10):1585-99. doi: 10.1093/pcp/pct103. Epub 2013 Jul 19.
Abstract
Brefeldin A suppresses vesicle trafficking by inhibiting exchange of GDP for GTP in ADP-ribosylation factor. We report that brefeldin A suppresses mobilization of triacylglycerols in Chlamydomonas reinhardtii, a model organism of green microalgae. Analyses revealed that brefeldin A causes Chlamydomonas to form lipid droplets in which triacylglycerols accumulate in a dose-dependent manner. Pulse labeling experiment using fluorescent fatty acids suggested that brefeldin A inhibits the cells from degrading fatty acids. The experiment also revealed that the cells transiently form novel compartments that accumulate exogenously added fatty acids in the cytoplasm, designated fatty acid-induced microbodies (FAIMs). Brefeldin A up-regulates the formation of FAIMs, whereas nitrogen deprivation that up-regulates triacylglycerol synthesis in Chlamydomonas does not cause the cells to form FAIMs. These results underscore the role of the vesicle trafficking machinery in triacylglycerol metabolism in green microalgae.
摘要
布雷菲德菌素 A 通过抑制 ADP-核糖基化因子 GDP 与 GTP 的交换来抑制囊泡转运。我们报告称，布雷菲德菌素 A 抑制了莱茵衣藻（一种绿藻模式生物）中三酰基甘油的动员。分析表明，布雷菲德菌素 A 导致衣藻形成脂质滴，其中三酰基甘油以剂量依赖的方式积累。使用荧光脂肪酸的脉冲标记实验表明，布雷菲德菌素 A 抑制细胞降解脂肪酸。该实验还表明，细胞会短暂形成新的隔室，在细胞质中积累外加的脂肪酸，这些隔室被命名为脂肪酸诱导的微体（FAIMs）。布雷菲德菌素 A 上调 FAIM 的形成，而氮饥饿会在上调莱茵衣藻三酰基甘油合成的同时不会导致细胞形成 FAIM。这些结果强调了囊泡转运机制在绿藻三酰基甘油代谢中的作用。
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