School of Bioengineering, Dalian University of Technology, Dalian 116024, China.
Division of Biotechnology, The Catholic University of Korea, Bucheon 14662, Korea.
J Agric Food Chem. 2023 Nov 22;71(46):17833-17841. doi: 10.1021/acs.jafc.3c05290. Epub 2023 Nov 7.
Microalgae are promising platforms for biofuel production. Transcription factors (TFs) are emerging as key regulators of lipid metabolism for biofuel production in microalgae. We previously identified a novel TF MYB1, which mediates lipid accumulation in the green microalga under nitrogen depletion. However, the function of on lipid metabolism in microalgae under standard growth conditions remains poorly understood. Here, we examined the effects of overexpression (OE) on lipid metabolism and physiological changes in . Under standard growth conditions, OE transformants accumulated 1.9 to 3.2-fold more triacylglycerols (TAGs) than that in the parental line (PL), and total fatty acids (FAs) also significantly increased. Moreover, saturated FA (C16:0) was enriched in TAGs and total FAs in OE transformants. Notably, starch and protein content and biomass production also significantly increased in OE transformants compared with that in PL. Furthermore, RT-qPCR results showed that the expressions of key genes involved in TAG, FA, and starch biosynthesis were upregulated. In addition, OE transformants showed higher biomass production without a compromised cell growth rate and photosynthetic activity. Overall, our results indicate that overexpression not only enhanced lipid content but also improved starch and protein content and biomass production under standard growth conditions. TF MYB1 engineering is a promising genetic engineering tool for biofuel production in microalgae.
微藻是生物燃料生产的有前途的平台。转录因子（TFs）作为微藻生物燃料生产中脂质代谢的关键调节剂正在出现。我们之前鉴定了一种新型 TF MYB1，它介导绿色微藻在氮饥饿下的脂质积累。然而，在标准生长条件下， 对微藻脂质代谢的功能仍知之甚少。在这里，我们研究了过表达（OE）对 的脂质代谢和生理变化的影响。在标准生长条件下，OE 转化体比亲本系（PL）积累的三酰基甘油（TAG）多 1.9 到 3.2 倍，总脂肪酸（FAs）也显著增加。此外，TAG 和总 FA 中的饱和 FA（C16:0）含量丰富。值得注意的是，与 PL 相比，OE 转化体中的淀粉和蛋白质含量以及生物量产量也显著增加。此外，RT-qPCR 结果表明，参与 TAG、FA 和淀粉生物合成的关键基因的表达上调。此外，OE 转化体表现出更高的生物量产量，而细胞生长速率和光合活性不受影响。总的来说，我们的结果表明， 过表达不仅可以提高脂质含量，还可以在标准生长条件下提高淀粉和蛋白质含量以及生物量产量。TF MYB1 工程是微藻生物燃料生产中一种很有前途的遗传工程工具。
