Department of Animal Biotechnology and Histology, University of Technology and Life Sciences in Bydgoszcz, Mazowiecka 28, 85-225, Bydgoszcz, Poland,
J Appl Genet. 2013 Nov;54(4):489-92. doi: 10.1007/s13353-013-0161-1. Epub 2013 Jul 20.
Toll-like receptors (TLRs) are members of the cellular receptors that constitute a major component of the evolutionary conserved pattern recognition system (PRR). TLRs are expressed in a wide variety of tissues and cell types. In this study we compared the expression profiles of the chicken TLR1, TLR2 and TLR5 genes in a range of organs (lung, ovary, liver, thymus, duodenum, spleen and large intestine) in commercial Hy-Line (HL) and indigenous Green-legged Partridgelike (GP) chickens. The level of mRNA was determined with RT-qPCR using the TaqMan probes for target and reference (ACTB) genes. We determined that the tissue profiles differed with respect to each TLR and they were ranked as follows: spleen, lungs, large intestine (TLR1), large intestine, lungs, thymus/ovary (TLR2) and lungs, thymus, liver (TLR5). A differential expression between HL and GP chickens was determined for TLR1 and TLR5 genes in large intestine and thymus of HL (P < 0.05) and GP (P < 0.05) chickens. We conclude that the commercial chickens expressed higher levels of TLR1 mRNA in large intestine and TLR5 mRNA in thymus than indigenous chickens.
Toll 样受体 (TLRs) 是细胞受体的成员，构成了进化保守的模式识别系统 (PRR) 的主要组成部分。TLRs 在广泛的组织和细胞类型中表达。在这项研究中，我们比较了 TLR1、TLR2 和 TLR5 基因在商业海兰 (HL) 和本地绿脚鹧鸪样 (GP) 鸡的一系列器官 (肺、卵巢、肝、胸腺、十二指肠、脾和大肠) 中的表达谱。使用 TaqMan 探针检测 TLR1、TLR2 和 TLR5 基因的靶标和参考 (ACTB) 基因的 RT-qPCR 来确定 mRNA 水平。我们确定组织谱因每个 TLR 而异，其排序如下：脾、肺、大肠 (TLR1)、大肠、肺、胸腺/卵巢 (TLR2) 和肺、胸腺、肝 (TLR5)。在 HL 和 GP 鸡的大肠和胸腺中，TLR1 和 TLR5 基因的 HL (P < 0.05) 和 GP (P < 0.05) 鸡之间存在差异表达。我们得出结论，商业鸡在大肠中表达更高水平的 TLR1 mRNA，在胸腺中表达更高水平的 TLR5 mRNA 比本地鸡。
