Department of Physical Therapy, Nagoya University Graduate School of Medicine, 1-1-20 Daiko-minami, Higashi-ku, Nagoya, Aichi 461-8673, Japan ; Okinawa Rehabilitation Center Hospital, 2-15-1 Hiyagon, Okinawa, Okinawa 904-2173, Japan.
J Clin Biochem Nutr. 2013 Jul;53(1):8-14. doi: 10.3164/jcbn.12-72. Epub 2013 Apr 9.
Long-term exercise prior to brain ischemia enhances the activities of antioxidant enzymes and leads to a significant reduction in brain damage and neurological deficits in rats subjected to transient middle cerebral artery occlusion. However, it has not been established whether relatively short-term exercise generates similar results following middle cerebral artery occlusion. We aimed to determine whether short-term exercise could reduce oxidative damage and prevent sensori-motor dysfunction. Male Wistar rats were subjected to perform daily exercise on a treadmill for 30 min at a speed of 15 m/min for 3 weeks, followed by a 90-min middle cerebral artery occlusion. Animals were assessed after middle cerebral artery occlusion for neurological deficits and sensori-motor function. Brain tissues were processed to evaluate infarct volume and oxidative damage. Oxidative stress was assessed using immunohistochemistry for 4-hydroxy-2-nonenal-modified proteins and 8-hydroxy-2'-deoxyguanosine. Antioxidant enzymes were evaluated using immunohistochemistry for thioredoxin and activity assay for superoxide dismutase. Exercise for 3 weeks decreased the severity of paralysis and impairment in forelimb motor coordination. Furthermore, exercise had effect on superoxide dismutase and reduced the infarct volume and the number of cells immunopositive for 4-hydroxy-2-nonenal-modified proteins and 8-hydroxy-2'-deoxyguanosine. Our results suggest that pre-conditioning treadmill exercise for 3 weeks is useful for ameliorating ischemia-induced brain injury.
长期运动预处理脑缺血可增强抗氧化酶的活性，显著减少大鼠短暂性大脑中动脉闭塞后的脑损伤和神经功能缺损。然而，尚未确定相对短期运动是否会在大脑中动脉闭塞后产生类似的结果。我们旨在确定短期运动是否可以减轻氧化损伤并预防感觉运动功能障碍。雄性 Wistar 大鼠每天在跑步机上以 15m/min 的速度运动 30min，持续 3 周，然后进行 90min 的大脑中动脉闭塞。在大脑中动脉闭塞后，对动物进行神经功能缺损和感觉运动功能评估。处理脑组织以评估梗死体积和氧化损伤。使用 4-羟基-2-壬烯醛修饰蛋白和 8-羟基-2'-脱氧鸟苷的免疫组织化学评估氧化应激。使用硫氧还蛋白的免疫组织化学和超氧化物歧化酶的活性测定评估抗氧化酶。3 周的运动降低了瘫痪和前肢运动协调障碍的严重程度。此外，运动对超氧化物歧化酶有影响，减少了梗死体积和对 4-羟基-2-壬烯醛修饰蛋白和 8-羟基-2'-脱氧鸟苷呈免疫阳性的细胞数量。我们的结果表明，3 周的跑步机预适应运动有助于改善缺血性脑损伤。
