Department of Respiratory Medicine, The Fourth Hospital of Hebei Medical University, Shijiazhuang, People's Republic of China.
Onco Targets Ther. 2013 Jul 11;6:877-81. doi: 10.2147/OTT.S48284. Print 2013.
MicroRNA (miRNA)-related single-nucleotide polymorphisms (SNPs) in miRNA processing machinery genes can affect cancer risk, treatment efficacy, and patient prognosis. A miR-SNP of rs11077 located in the 3' untranslated region (3' UTR) of the miRNA processing machinery gene XPO5 was examined in 112 advanced non-small-cell lung cancer (NSCLC) patients to evaluate its association with cancer outcome.
The miR-SNP was genotyped with ligase detection reaction method. Survival curves were calculated using the Kaplan-Meier method, and multivariate survival analysis was performed using a Cox proportional hazards model.
The AC genotype of rs11077, which carries C or A allele, was significantly associated with a better chemotherapy response (P = 0.001). In addition, rs11077 was independently associated with overall survival in advanced NSCLC patients through multivariate analysis (relative risk 0.457; 95% confidence interval: 0.251-0.831; P = 0.010).
rs11077 was associated with chemotherapy response and survival of advanced NSCLC patients. The analysis of miR-SNPs in miRNA processing machinery genes can help identify patient subgroups that are at high risk for poor disease outcomes.
miRNA 处理机制基因中的 miRNA 相关单核苷酸多态性（SNP）可影响癌症风险、治疗效果和患者预后。本研究检测了 miRNA 处理机制基因 XPO5 的 3'非翻译区（3'UTR）中的 miR-SNP rs11077，评估其与癌症结局的相关性。
采用连接酶检测反应法对 miR-SNP 进行基因分型。采用 Kaplan-Meier 法计算生存曲线，Cox 比例风险模型进行多因素生存分析。
rs11077 的 AC 基因型（携带 C 或 A 等位基因）与化疗反应良好显著相关（P = 0.001）。此外，通过多因素分析，rs11077 与晚期 NSCLC 患者的总生存独立相关（相对风险 0.457；95%置信区间：0.251-0.831；P = 0.010）。
rs11077 与晚期 NSCLC 患者的化疗反应和生存相关。分析 miRNA 处理机制基因中的 miR-SNPs 有助于识别疾病结局不良风险较高的患者亚组。
