Department of Nutrition and Health Sciences, University of Nebraska-Lincoln, 211 Ruth Leverton Hall, Lincoln, NE 68583-0806, USA.
J Int Soc Sports Nutr. 2013 Jul 22;10:33. doi: 10.1186/1550-2783-10-33. eCollection 2013.
Anatabine (ANA), a minor tobacco alkaloid found in the Solanaceae family of plants, may exhibit anti-inflammatory activity, which may be useful to aid in recovery from exercise-induced muscle damage. The purpose of this study, therefore, was to examine the effects of ANA supplementation on the recovery of isometric strength and selected non-invasive indicators of muscle damage.
A double-blinded, placebo-controlled, crossover design was used to study eighteen men (mean ± SD age = 22.2 ± 3.1 yrs; body mass = 80.3 ± 15.7 kg) who participated in two randomly-ordered conditions separated by a washout period. The ANA condition consisted of consuming 6-12 mg anatabine per day for 10 days, while testing took place during days 7-10. The placebo (PLA) condition was identical except that the PLA supplement contained no ANA. Maximal voluntary isometric peak torque (PT) of the forearm flexors, arm circumference, hanging joint angle, and subjective pain ratings were measured before (PRE), immediately after (POST), and 24, 48, and 72 h after six sets of 10 maximal, eccentric isokinetic forearm flexion muscle actions. Resting heart rate and blood pressure were measured at PRE and 72 h in each condition.
For PT, hanging joint angle, arm circumference, and subjective pain ratings, there were no condition x time (p > 0.05) interactions, there were no main effects for condition (p > 0.05), but there were main effects for time (p < 0.001). There were no condition x time (p > 0.05) interactions and no main effects for condition (p > 0.05) or time (p > 0.05) for blood pressure or resting heart rate.
ANA supplementation had no effect on the recovery of muscle strength, hanging joint angle, arm swelling, or subjective pain ratings after a bout of maximal eccentric exercise in the forearm flexors. Therefore, ANA may not be beneficial for those seeking to improve recovery from heavy eccentric exercise. Future studies should examine the effects of ANA on the pro-inflammatory cytokine responses to exercise-induced muscle damage and the chronic low-grade inflammation observed in obese and elderly individuals.
安纳托宾（ANA）是茄科植物中发现的一种次要烟草生物碱，可能具有抗炎活性，这可能有助于从运动引起的肌肉损伤中恢复。因此，本研究的目的是检查 ANA 补充对等长强度恢复和选定的非侵入性肌肉损伤指标的影响。
采用双盲、安慰剂对照、交叉设计研究 18 名男性（平均年龄±标准差 22.2±3.1 岁；体重 80.3±15.7 公斤），他们参加了两个随机顺序的条件，间隔洗脱期。ANA 条件包括每天摄入 6-12 毫克安纳托宾 10 天，而测试在第 7-10 天进行。安慰剂（PLA）条件相同，只是 PLA 补充剂不含 ANA。在进行 6 组 10 次最大、离心等速前臂屈肌运动后，立即测量前臂屈肌最大自愿等长峰值扭矩（PT）、臂围、悬挂关节角度和主观疼痛评分。在每个条件下，在 PRE 和 72 小时测量静息心率和血压。
对于 PT、悬挂关节角度、臂围和主观疼痛评分，条件 x 时间无交互作用（p>0.05），条件无主效应（p>0.05），但时间有主效应（p<0.001）。血压或静息心率无条件 x 时间交互作用（p>0.05），无条件主效应（p>0.05）或时间主效应（p>0.05）。
ANA 补充剂对最大离心运动后前臂屈肌力量、悬挂关节角度、手臂肿胀或主观疼痛评分的恢复没有影响。因此，ANA 可能对那些试图从剧烈离心运动中恢复的人没有好处。未来的研究应该检查 ANA 对运动引起的肌肉损伤的促炎细胞因子反应以及肥胖和老年人中观察到的慢性低度炎症的影响。
