The Education Ministry Key Laboratory of Resource Chemistry and Shanghai Key Laboratory of Rare Earth Functional Materials, Department of Chemistry, College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, PR China.
Biosens Bioelectron. 2013 Dec 15;50:300-4. doi: 10.1016/j.bios.2013.06.045. Epub 2013 Jul 8.
A novel electrochemiluminescence (ECL) strategy based on the sandwich-type immunosensor for sensitive detection of retinol-binding protein (RBP) was developed. The primary antibody anti-RBP was immobilized onto multiwalled carbon nanotubes (MWCNTs), which have large surface area and high electrical conductivity. The RBP antigen and Ru-Nafion@SiO2-labeled secondary antibody were then successively conjugated to form sandwich-type immunocomplexes through the specific interaction between antigen and antibody. The ECL signal amplification was significantly improved due to the synergistic effect of MWCNTs and mesoporous silica nanospheres (mSiO2). The developed ECL immunosensor exhibited high sensitivity and specificity for the detection of RBP and responded linearly to the clinically-relevant concentration of RBP from 78 to 5000 ng mL(-1). Moreover, the MWCNT-based ECL immunosensor displayed excellent stability and reproducibility, as well as successfully achieved the detection of RBP in patient urine samples with desirable results. The present work provided a promising technique for the clinical screening of RBP and point-of-care diagnostics.
一种基于三明治型免疫传感器的新型电化学发光（ECL）策略被开发出来，用于灵敏检测视黄醇结合蛋白（RBP）。将抗 RBP 抗体固定在具有大表面积和高导电性的多壁碳纳米管（MWCNTs）上。然后，RBP 抗原和 Ru-Nafion@SiO2 标记的二抗通过抗原和抗体之间的特异性相互作用，相继被共轭形成三明治型免疫复合物。由于 MWCNTs 和介孔硅纳米球（mSiO2）的协同作用，ECL 信号得到了显著增强。所开发的 ECL 免疫传感器对 RBP 的检测具有高灵敏度和特异性，对临床相关浓度的 RBP 具有从 78 到 5000ngmL-1 的线性响应。此外，基于 MWCNT 的 ECL 免疫传感器表现出优异的稳定性和重现性，并成功地实现了对患者尿液样本中 RBP 的检测，结果令人满意。本工作为 RBP 的临床筛选和即时诊断提供了一种很有前途的技术。
