Li Linzhi, Liu Xing, He Saijun, Cao Hongmei, Su Benchao, Huang Tianzeng, Chen Qi, Liu Minghuan, Yang Da-Peng
College of Food Science and Technology, Hainan University, 58 Renmin Avenue, Haikou 570228, China.
College of Chemistry and Engineering Technology, Hainan University, 58 Renmin Avenue, Haikou 570228, China.
ACS Omega. 2021 Oct 28;6(44):30148-30156. doi: 10.1021/acsomega.1c05213. eCollection 2021 Nov 9.
A novel ultrasensitive electrochemiluminescence (ECL) immunoassay based on Au/CaCO was proposed for detecting ochratoxin A (OTA) in coffee. Au/CaCO nanocomposites synthesized using waste eggshells as the template with a large surface area and excellent electrochemical properties were applied for immobilizing a large amount of Ru(bpy) and conjugating a high-affinity nanobody (prepared by the phage display technique). Coupling of the Au/CaCO nanocomposites and nanobody technologies provided an ultrasensitive and highly selective ECL immunosensor for OTA detection in the range of 10 pg/mL-100 ng/mL with a low detection limit of 5.7 pg/mL. Moreover, the as-prepared ECL immunosensor showed excellent performance and high stability. Finally, the proposed ECL sensor was applied to analyze OTA in coffee samples, confirming the desirable accuracy and practical applicability potential. Overall, this work presents a new nanomaterial for fabricating the sensing interface of immunosensors by harnessing natural waste as the source and a method for detecting toxic OTA in foods.
提出了一种基于金/碳酸钙的新型超灵敏电化学发光（ECL）免疫分析法，用于检测咖啡中的赭曲霉毒素A（OTA）。以废弃蛋壳为模板合成的具有大表面积和优异电化学性能的金/碳酸钙纳米复合材料，用于固定大量的钌（联吡啶）并偶联高亲和力纳米抗体（通过噬菌体展示技术制备）。金/碳酸钙纳米复合材料与纳米抗体技术的结合提供了一种超灵敏且高选择性的ECL免疫传感器，用于检测OTA，检测范围为10 pg/mL至100 ng/mL，检测限低至5.7 pg/mL。此外，所制备的ECL免疫传感器表现出优异的性能和高稳定性。最后，将所提出的ECL传感器应用于分析咖啡样品中的OTA，证实了其良好的准确性和实际应用潜力。总体而言，这项工作提出了一种利用天然废弃物作为来源制造免疫传感器传感界面的新型纳米材料，以及一种检测食品中有毒OTA的方法。
