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采用 HPLC 法对续断药材中生物活性标志物化合物进行定量测定和模式识别分析。

Quantitative determination and pattern recognition analyses of bioactive marker compounds from Dipsaci Radix by HPLC.

机构信息

College of Pharmacy, Catholic University of Daegu, Gyeongsan, 712-702, Korea.

出版信息

Arch Pharm Res. 2013 Nov;36(11):1345-53. doi: 10.1007/s12272-013-0162-y. Epub 2013 Jul 23.

DOI:10.1007/s12272-013-0162-y
PMID:23877237
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3825280/
Abstract

In this study, quantitative and pattern recognition analyses were developed using HPLC/UV for the quality evaluation of Dipsaci Radix. For quantitative analysis, five major bioactive compounds were assessed. The separation conditions employed for HPLC/UV were optimized using ODS C18 column (250 × 4.6 mm, 5 μm) with a gradient of acetonitrile and water as the mobile phase at a flow rate of 1.0 mL/min and a detection wavelength of 212 nm. These methods were fully validated with respect to linearity, accuracy, precision, recovery, and robustness. The HPLC/UV method was applied successfully to the quantification of five major compounds in the extract of Dipsaci Radix. The HPLC analytical method for pattern recognition analysis was validated by repeated analysis of 17 Dipsaci Radix and four Phlomidis Radix samples. The results indicate that the established HPLC/UV method is suitable for quantitative analysis.

摘要

本研究采用 HPLC/UV 对续断药材进行定量和模式识别分析，用于质量评价。定量分析评估了五种主要生物活性化合物。采用 ODS C18 柱（250×4.6mm，5μm），以乙腈和水为流动相，梯度洗脱，流速为 1.0mL/min，检测波长为 212nm，优化了 HPLC/UV 的分离条件。这些方法在线性、准确性、精密度、回收率和稳健性方面均得到了充分验证。HPLC/UV 法成功应用于续断药材提取物中五种主要化合物的定量分析。模式识别分析的 HPLC 分析方法通过重复分析 17 份续断和 4 份Phlomidis Radix 样品进行了验证。结果表明，建立的 HPLC/UV 方法适用于定量分析。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a388/3825280/8fd812f3af1e/12272_2013_162_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a388/3825280/015ebc9d5118/12272_2013_162_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a388/3825280/444cdf73ed11/12272_2013_162_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a388/3825280/8fd812f3af1e/12272_2013_162_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a388/3825280/015ebc9d5118/12272_2013_162_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a388/3825280/444cdf73ed11/12272_2013_162_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a388/3825280/8fd812f3af1e/12272_2013_162_Fig3_HTML.jpg

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