Chun Jin Mi, Lee A Yeong, Nam Jae Yong, Lim Kyung Seob, Choe Mu Seog, Lee Min Young, Kim Chul, Kim Joong-Sun
Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine, Naju-si, Korea.
Bioinformatics Group, R&D Center, Insilicogen Corporation, Yongin, Korea.
Front Pharmacol. 2021 Apr 13;12:615157. doi: 10.3389/fphar.2021.615157. eCollection 2021.
The root of C. Y. Cheng et T. M. Ai is traditionally used as an analgesic and anti-inflammatory agent to treat pain, rheumatoid arthritis, and bone fractures. However, neither its effects on osteoarthritis (OA) nor its effects on the arthritic cartilage tissue transcriptome have not been fully investigated. In this study, we used a rat model of monosodium iodoacetate- (MIA-) induced OA to investigate the therapeutic effects of a ethanolic extract (DAE, 200 mg/kg for 21 days). The study first assessed joint diameter, micro-CT scans, and histopathological analysis and then conducted gene expression profiling using RNA sequencing in articular cartilage tissue. We found that DAE treatment ameliorates OA disease phenotypes; it reduced the knee joint diameter and prevented changes in the structural and histological features of the joint, thereby showing that DAE has a protective effect against OA. Based on the results of gene expression profiling and subsequent pathway analysis, we found that several canonical pathways were linked to DAE treatment, including WNT/β-catenin signaling. Taken together, the present results suggest molecular mechanism, involving gene expression changes, by which DAE has a protective effect in a rat model of MIA-induced OA.
传统上，城口金粟兰（C. Y. Cheng et T. M. Ai）的根被用作止痛和抗炎剂，用于治疗疼痛、类风湿性关节炎和骨折。然而，其对骨关节炎（OA）的影响以及对关节炎软骨组织转录组的影响均未得到充分研究。在本研究中，我们使用碘乙酸钠（MIA）诱导的OA大鼠模型，研究了乙醇提取物（DAE，200 mg/kg，持续21天）的治疗效果。该研究首先评估了关节直径、显微CT扫描和组织病理学分析，然后在关节软骨组织中使用RNA测序进行基因表达谱分析。我们发现，DAE治疗可改善OA疾病表型；它减小了膝关节直径，并防止了关节结构和组织学特征的变化，从而表明DAE对OA具有保护作用。基于基因表达谱分析结果和随后的通路分析，我们发现几个经典通路与DAE治疗有关，包括WNT/β-连环蛋白信号通路。综上所述，目前的结果表明了DAE在MIA诱导的OA大鼠模型中发挥保护作用的分子机制，其中涉及基因表达变化。
