Midgley J M, Andrew R, Watson D G, MacDonald N, Reid J L, Williams D A
Department of Pharmacy, University of Strathclyde, Glasgow, U.K.
J Chromatogr. 1990 May 18;527(2):259-65. doi: 10.1016/s0378-4347(00)82110-6.
Dichlorotetrafluoroacetone has been used to prepare 4-substituted 2-bis (chlorodifluoromethyl)-1,3-dioxolan-5-one derivatives of mandelic acids which were found to be suitable for the analysis of these compounds by gas chromatography-negative-ion chemical ionisation mass spectrometry (GC-NICIMS). The high specificity of the derivatising agent facilitates the identification and quantitation of small amounts of mandelic acids in complex biological matrices. The derivatisation procedure was used to determine the concentrations of m- and p-hydroxymandelic acids and vanillylmandelic acid in to the urine of normal subjects. The method may also have application in the determination of isomeric phenylethylene glycols, the corresponding products of reductive metabolism of biogenic amines.
二氯四氟丙酮已被用于制备扁桃酸的4-取代-2-双（氯二氟甲基）-1,3-二氧戊环-5-酮衍生物，发现这些衍生物适用于通过气相色谱-负离子化学电离质谱法（GC-NICIMS）分析这些化合物。衍生剂的高特异性有助于在复杂生物基质中鉴定和定量少量扁桃酸。该衍生化程序用于测定正常受试者尿液中间羟基扁桃酸、对羟基扁桃酸和香草扁桃酸的浓度。该方法也可能应用于生物胺还原代谢的相应产物——异构苯乙二醇的测定。
