Midgley J M, Couch M W, Crowley J R, Williams C M
Biomed Mass Spectrom. 1979 Nov;6(11):485-90. doi: 10.1002/bms.1200061106.
o-Hydroxymandelic acid and m-hydroxymandelic acid have been identified in human urine by gas chromatography mass spectrometry selected ion monitoring. After solvent extraction the urinary acids were converted to their O-trifluoroacetoxy methyl ester derivatives which were identified by comparison of the retention times and relative intensities of the characteristic m/z 374 and m/z 315 ions with those from authentic samples. 4,6-[2H3]-o-hydroxymandelic acid and 2,4,6-[2H3]-m-hydroxymandelic acid were synthesized for use as internal standards in the quantitative estimation of the isomeric hydroxymandelic acids excreted in urine. In ten normal adults the following results were obtained: o-hydroxymandelic acid 4--16 ng mg-1 creatinine and m-hydroxymandelic acid 11--71 ng mg-1 creatinine. Acid hydrolysis of the urine or ingestion of a diet of known composition did not affect these results, indicating that these compounds are excreted as the free acids and probably arise by metabolism of the corresponding phenylethanolamine(s).
已通过气相色谱-质谱选择离子监测法在人尿中鉴定出邻羟基扁桃酸和间羟基扁桃酸。溶剂萃取后，将尿中的酸转化为它们的O-三氟乙酰氧基甲酯衍生物，通过比较保留时间以及特征性m/z 374和m/z 315离子的相对强度与来自真实样品的离子来进行鉴定。合成了4,6-[2H₃]-邻羟基扁桃酸和2,4,6-[2H₃]-间羟基扁桃酸，用作定量估算尿中排泄的异构羟基扁桃酸的内标物。在十名正常成年人中获得了以下结果：邻羟基扁桃酸为4--16 ng mg⁻¹肌酐，间羟基扁桃酸为11--71 ng mg⁻¹肌酐。尿液的酸水解或摄入已知成分的饮食均未影响这些结果，表明这些化合物以游离酸形式排泄，可能是由相应的苯乙醇胺代谢产生的。
