Higa S, Kishimoto S
Anal Biochem. 1986 Apr;154(1):71-4. doi: 10.1016/0003-2697(86)90497-5.
Boric acid has been known to make a complex with 2-hydroxycarboxylic acids. Based on this principle, we have developed a new method for isolation of vanillylmandelic acid, p-hydroxymandelic acid, vanillyllactic acid, and p-hydroxyphenyllactic acid in urine. The technique involves two steps: extraction of lipophilic compounds from urine with n-butanol and selective isolation of 2-hydroxy acids in the n-butanol phase on a phenylboronate gel column. 2-Hydroxy acids were eluted from the column with 1.5 ml of 2% acetyl chloride in methanol with the recoveries of ca. 70%. According to analysis of urinary extract by gas chromatography--mass spectrometry, the mean excretion rates of the above four compounds in 12 healthy subjects were 3.68, 1.93, 0.091, and 1.08 mg/day, respectively.
已知硼酸可与2-羟基羧酸形成络合物。基于这一原理，我们开发了一种新方法，用于分离尿液中的香草扁桃酸、对羟基扁桃酸、香草乳酸和对羟基苯乳酸。该技术包括两个步骤：用正丁醇从尿液中萃取亲脂性化合物，以及在苯基硼酸酯凝胶柱上从正丁醇相中选择性分离2-羟基酸。用1.5毫升2%乙酰氯的甲醇溶液从柱上洗脱2-羟基酸，回收率约为70%。根据气相色谱-质谱法对尿液提取物的分析，12名健康受试者中上述四种化合物的平均排泄率分别为3.68、1.93、0.091和1.08毫克/天。
