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斑纹鳐(Raja pulchra)(鳐形目,鳐科)的完整线粒体基因组

Complete mitochondrial genome of the mottled skate: Raja pulchra (Rajiformes, Rajidae).

作者信息

Jeong Dageum, Kim Sung, Kim Choong-Gon, Myoung Jung-Goo, Lee Youn-Ho

机构信息

a Marine Ecosystem Research Division , Korea Institute of Ocean Science & Technology , Ansan , Republic of Korea .

b Department of Marine Biology , University of Science and Technology , Daejeon , Republic of Korea , and.

出版信息

Mitochondrial DNA A DNA Mapp Seq Anal. 2016 May;27(3):1823-4. doi: 10.3109/19401736.2014.971242. Epub 2014 Oct 15.

DOI:10.3109/19401736.2014.971242
PMID:25317638
Abstract

The complete sequence of mitochondrial DNA of a mottled skate, Raja pulchra was sequenced as being circular molecules of 16,907 bp including 2 rRNA, 22 tRNA, 13 protein-coding genes (PCGs), and an AT-rich control region. The organization of the PCGs is the same as those found in other Rajidae species. The nucleotide of L-strand is composed of 29.8% A, 28.0% C, 27.9% T, and 14.3% G with a bias toward A + T slightly. Twelve of 13 PCGs are initiated by the ATG codon while COX1 starts with GTG. Only ND4 harbors the incomplete termination codon, TA. All tRNA genes have a typical clover-leaf structure of mitochondrial tRNA with the exception of [Formula: see text] which has a reduced DHU arm. This mitogenome will provide essential information for better phylogenetic resolution and precision of the family Rajidae and the genus Raja as well as for establishment of a fish stock recovery plan of the species.

摘要

斑纹鳐(Raja pulchra)线粒体DNA的完整序列被测序为16,907 bp的环状分子,包括2个rRNA、22个tRNA、13个蛋白质编码基因(PCGs)和一个富含AT的控制区。PCGs的组织方式与其他鳐科物种相同。轻链的核苷酸由29.8%的A、28.0%的C、27.9%的T和14.3%的G组成,略微偏向于A+T。13个PCGs中有12个由ATG密码子起始,而COX1以GTG起始。只有ND4含有不完全终止密码子TA。除了[公式:见正文]具有缩短的DHU臂外,所有tRNA基因都具有典型的线粒体tRNA三叶草结构。这个线粒体基因组将为更好地解决鳐科和鳐属的系统发育分辨率和精度以及制定该物种的鱼类种群恢复计划提供重要信息。

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