通过5-氮杂胞苷诱导普通小麦中增强染色体畸变的杀配子染色体。

Gametocidal chromosomes enhancing chromosome aberration in common wheat induced by 5-azacytidine.

作者信息

Su W-Y, Cong W-W, Shu Y-J, Wang D, Xu G-H, Guo C-H

机构信息

Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province, College of Life Science and Technology, Harbin Normal University, Harbin, Heilongjiang Province, China.

出版信息

Genet Mol Res. 2013 Jul 8;12(3):2227-33. doi: 10.4238/2013.July.8.4.

DOI:10.4238/2013.July.8.4

PMID:23884766

Abstract

The gametocidal (Gc) chromosome from Aegilops spp induces chromosome mutation, which is introduced into common wheat as a tool of chromosome manipulation for genetic improvement. The Gc chromosome functions similar to a restriction-modification system in bacteria, in which DNA methylation is an important regulator. We treated root tips of wheat carrying Gc chromosomes with the hypomethylation agent 5-azacytidine; chromosome breakage and micronuclei were observed in these root tips. The frequency of aberrations differed in wheat containing different Gc chromosomes, suggesting different functions inducing chromosome breakage. Gc chromosome 3C caused the greatest degree of chromosome aberration, while Gc chromosome 3C(SAT) and 2C caused only slight chromosome aberration. Gc chromosome 3C induced different degrees of chromosome aberration in wheat varieties Triticum aestivum var. Chinese Spring and Norin 26, demonstrating an inhibition function in common wheat.

摘要

来自节节麦属的杀配子（Gc）染色体可诱导染色体突变，作为一种染色体操作工具被导入普通小麦以进行遗传改良。Gc染色体的功能类似于细菌中的限制修饰系统，其中DNA甲基化是一个重要的调节因子。我们用去甲基化剂5-氮杂胞苷处理携带Gc染色体的小麦根尖；在这些根尖中观察到了染色体断裂和微核。含有不同Gc染色体的小麦中畸变频率不同，表明诱导染色体断裂的功能不同。3C Gc染色体导致的染色体畸变程度最大，而3C（SAT）Gc染色体和2C Gc染色体仅导致轻微的染色体畸变。3C Gc染色体在普通小麦品种中国春和诺林26中诱导了不同程度的染色体畸变，表明其在普通小麦中具有抑制功能。