Wang Dan, Zhao Jieyu, Bai Yan, Ao You, Guo Changhong
Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province, College of Life Science and Technology, Harbin Normal University, No. 1 of Shida Road, Limin Development Zone, Harbin 150025, China.
Int J Mol Sci. 2017 Aug 10;18(8):1738. doi: 10.3390/ijms18081738.
Gametocidal (Gc) chromosomes can ensure their preferential transmission by killing the gametes without themselves through causing chromosome breakage and therefore have been exploited as an effective tool for genetic breeding. However, to date very little is known about the molecular mechanism of Gc action. In this study, we used methylation-sensitive amplified polymorphism (MSAP) technique to assess the extent and pattern of cytosine methylation alterations at the whole genome level between two lines of wheat Gc addition line and their common wheat parent. The results indicated that the overall levels of cytosine methylation of two studied Gc addition lines (CS-3C and CS-3C3C, 48.68% and 48.65%, respectively) were significantly increased when compared to common wheat CS (41.31%) and no matter fully methylated or hemimethylated rates enhanced in Gc addition lines. A set of 30 isolated fragments that showed different DNA methylation or demethylation patterns between the three lines were sequenced and the results indicated that 8 fragments showed significant homology to known sequences, of which three were homologous to MITE transposon (Miniature inverted-repeat transposable elements), LTR-retrotransposon and retrotransposon , respectively. Overall, our results showed that DNA methylation could play a role in the Gc action.
杀配子染色体（Gc）可通过导致染色体断裂来杀死不含自身的配子，从而确保其优先传递，因此已被用作遗传育种的有效工具。然而，迄今为止，关于Gc作用的分子机制知之甚少。在本研究中，我们使用甲基化敏感扩增多态性（MSAP）技术，评估了小麦Gc附加系与其普通小麦亲本之间全基因组水平上胞嘧啶甲基化改变的程度和模式。结果表明，与普通小麦CS（41.31%）相比，两个研究的Gc附加系（CS - 3C和CS - 3C3C，分别为48.68%和48.65%）的胞嘧啶甲基化总体水平显著升高，并且Gc附加系中无论是全甲基化率还是半甲基化率都有所提高。对在这三个品系之间表现出不同DNA甲基化或去甲基化模式的一组30个分离片段进行了测序，结果表明其中8个片段与已知序列具有显著同源性，其中三个分别与MITE转座子（微型反向重复转座元件）、LTR反转录转座子和反转录转座子同源。总体而言，我们的结果表明DNA甲基化可能在Gc作用中发挥作用。
