Eamens G J, Bailey G, Gonsalves J R, Jenkins C
NSW Department of Primary Industries, Elizabeth Macarthur Agricultural Institute, Camden, New South Wales, 2570, Australia.
Aust Vet J. 2013 Aug;91(8):332-40. doi: 10.1111/avj.12078.
To determine the distribution of theilerial types in eastern Australian cattle herds and their changing prevalence in regions of New South Wales (NSW).
Survey testing of herds in 2010-11 in Queensland (QLD), NSW and Victoria (VIC) where clinical theileriosis was not evident and ongoing surveillance in NSW through laboratory submissions.
Blood samples were tested by PCR targeting the Theileria orientalis p32 gene and positive tests were examined for the Ikeda, Chitose and Buffeli types. Survey samples from 516 cattle in 50 herds and diagnostic submissions from 434 suspect field cases in 116 herds were analysed.
In clinically normal survey cattle, T. orientalis prevalence was high (NSW 23.7%, QLD 56.8%, VIC 34.0%), with variability among regions of each state. Chitose was the most common and widespread type (19.1-43.7% per state), with Buffeli present in all states at a lower prevalence (10.8-24.8% per state). Ikeda was detected in three of five regions in QLD (North, South and South East; prevalence 3.4-15.4%), only one of the surveyed regions in NSW (North Coast; prevalence 74.2%) and in only one animal in VIC. Evidence of clinical disease and laboratory confirmation of Ikeda infection in diagnostic submissions were predominant in several NSW regions, with increasing numbers of affected herds particularly in the coastal Mid-Coast and Cumberland areas.
In those regions where prior evidence of theileriosis was uncommon, Ikeda infection was evident in a limited number of NSW regions and multiple regions in QLD. However, clinical disease has continued to become widespread in NSW and VIC, involving Ikeda strains in many regions.
确定澳大利亚东部牛群中泰勒虫类型的分布情况及其在新南威尔士州（NSW）各地区流行率的变化。
2010 - 2011年对昆士兰州（QLD）、新南威尔士州和维多利亚州（VIC）未出现明显临床泰勒虫病的牛群进行调查检测，并通过实验室送检对新南威尔士州进行持续监测。
采用针对东方泰勒虫p32基因的聚合酶链反应（PCR）检测血样，对阳性检测结果分析池田、千岁和布氏泰勒虫类型。分析了来自50个牛群的516头牛的调查样本以及来自116个牛群的434例疑似田间病例的诊断送检样本。
在临床正常的调查牛中，东方泰勒虫流行率较高（新南威尔士州23.7%，昆士兰州56.8%，维多利亚州34.0%），且各州内不同地区存在差异。千岁型是最常见、分布最广的类型（各州为19.1 - 43.7%），布氏型在所有州的流行率较低（各州为10.8 - 24.8%）。池田型在昆士兰州的五个地区中的三个（北部、南部和东南部；流行率3.4 - 15.4%）、新南威尔士州仅一个被调查地区（北海岸；流行率74.2%）以及维多利亚州仅一头牛中被检测到。在新南威尔士州的几个地区，诊断送检样本中临床疾病证据和池田感染的实验室确诊情况较为突出，受影响牛群数量不断增加，尤其是在沿海的中海岸和坎伯兰地区。
在先前泰勒虫病证据不常见的地区，新南威尔士州的少数地区和昆士兰州的多个地区出现了池田感染。然而，新南威尔士州和维多利亚州的临床疾病仍在继续广泛传播，许多地区都涉及池田菌株。
