Selim A M, Das M, Senapati S K, Jena G R, Mishra C, Mohanty B, Panda S K, Patra R C
Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt, and Resident of Veterinary Clinical Medicine, Department of Clinical Medicine, College of Veterinary Science and Animal Husbandry, Odisha University of Agriculture and Technology, Bhubaneshwar 751003, Odisha, India.
Department of Clinical Medicine, College of Veterinary Science and Animal Husbandry, Odisha University of Agriculture and Technology, Bhubaneshwar 751003, Odisha, India.
Iran J Vet Res. 2020 Fall;21(4):250-256.
is a tick-borne apicomplexan parasite that affects bovine and causes severe economic losses. Our study aimed to determine the molecular prevalence of infection in asymptomatic carrier cattle in Odisha, India, to study the association of potential risk factors with theileriosis, and to investigate the effect of the parasite infection on hematological parameters in naturally affected animals.
A total of 226 cattle blood samples were collected from seven districts of Odisha, India. Molecular diagnoses of tropical theileriosis by polymerase chain reaction (PCR), cloning, sequencing, and phylogenetic analysis of isolated parasites were performed. Potential risk factors were investigated by univariable and multivariable logistic regression statistical analysis. Hematological parameters were compared between positive and negative animals.
All animals included in our study were clinically normal, however, 54.86% (124/226) of examined animals were positive by PCR for . The multivariable logistic regression showed that contact with other cattle from different herds during grazing (P<0.0001; OR: 12.75; 95% CI: 5.21-31.21), previous history of clinical signs (P=0.002; OR: 3.31; 95% CI: 1.53-6.31), and frequency of a ectoparasiticides application pre year (P<0.0001; OR: 9.22; 95% CI: 3.03-28.09) were the potential risk factors for the occurrence of tropical theileriosis. Nucleotide sequence identity data demonstrated that strain (MN818858) Odisha shared homology of 99.6%, 99.49%, and 99.36% with Uttar Pradesh, India (MF346035), Bahrain (AF214797), and Hyderabad, India (MK034702), respectively.
This is the first study to gain insight into the molecular epidemiology, risk factors, phylogeny, and hematological analysis of asymptomatic infected cattle from India.
是一种蜱传播的顶复门寄生虫，可感染牛并造成严重经济损失。我们的研究旨在确定印度奥里萨邦无症状携带牛感染的分子流行率，研究潜在风险因素与泰勒虫病的关联，并调查寄生虫感染对自然感染动物血液学参数的影响。
从印度奥里萨邦的七个地区共采集了226份牛血样本。通过聚合酶链反应（PCR）、克隆、测序以及对分离出的寄生虫进行系统发育分析，对热带泰勒虫病进行分子诊断。通过单变量和多变量逻辑回归统计分析调查潜在风险因素。比较阳性和阴性动物的血液学参数。
我们研究中的所有动物临床均正常，然而，经PCR检测，54.86%（124/226）的受检动物呈阳性。多变量逻辑回归显示，放牧期间与来自不同牛群的其他牛接触（P<0.0001；OR：12.75；95%CI：5.21 - 31.21）、既往临床症状史（P = 0.002；OR：3.31；95%CI：1.53 - 6.31）以及每年外用杀寄生虫剂的频率（P<0.0001；OR：9.22；95%CI：3.03 - 28.09）是热带泰勒虫病发生的潜在风险因素。核苷酸序列同一性数据表明，奥里萨邦的菌株（MN818858）与印度北方邦（MF346035）、巴林（AF214797）和印度海得拉巴（MK034702）分别具有99.6%、99.49%和99.36%的同源性。
这是首次对来自印度的无症状感染牛的分子流行病学、风险因素、系统发育和血液学分析进行深入研究。
