成纤维细胞生长因子 8 在体外耳蜗螺旋神经节神经元突起生长中的作用。
Role of fibroblast growth factor 8 in neurite outgrowth from spiral ganglion neurons in vitro.
机构信息
Department of Neuroscience, University of Connecticut Health Center, Farmington, Connecticut 06030, USA.
出版信息
Brain Res. 2013 Sep 5;1529:39-45. doi: 10.1016/j.brainres.2013.07.030. Epub 2013 Jul 24.
Abstract
Many neurons degenerate after injuries resulting from overstimulation, drugs, genetic mutations, and aging. Although several growth factors and neurotrophins delay degeneration and promote regrowth of neural processes, the role of fibroblast growth factor 8 (FGF8) in mammalian spiral ganglion neurons (SGN) neurite outgrowth has not been examined. This study develops and uses SGN cell cultures suitable for experimental analysis, it investigates whether FGF8a and FGF8b isoforms affect the neurite outgrowth from SGN cultured in vitro. We found that both FGF8a and FGF8b promoted the outgrowth of neurites from cultured SGN. This response is mediated by FGF receptors and involves the activation of IκBα-mediated NFκB signaling pathway. These findings suggest that, besides its morphogenetic role during development, FGF8 may have trophic functions in the adult which are relevant to regeneration.
摘要
许多神经元在过度刺激、药物、基因突变和衰老等导致的损伤后会发生退化。虽然几种生长因子和神经营养因子可以延缓退化并促进神经过程的再生，但成纤维细胞生长因子 8（FGF8）在哺乳动物螺旋神经节神经元（SGN）轴突生长中的作用尚未被研究过。本研究开发并使用了适合实验分析的 SGN 细胞培养物，研究了 FGF8a 和 FGF8b 同工型是否影响体外培养的 SGN 神经元的轴突生长。我们发现，FGF8a 和 FGF8b 都能促进体外培养的 SGN 神经元的轴突生长。这种反应是由 FGF 受体介导的，涉及 IκBα 介导的 NFκB 信号通路的激活。这些发现表明，除了在发育过程中的形态发生作用外，FGF8 在成年期可能具有营养作用，与再生有关。
相似文献
1
Role of fibroblast growth factor 8 in neurite outgrowth from spiral ganglion neurons in vitro.成纤维细胞生长因子 8 在体外耳蜗螺旋神经节神经元突起生长中的作用。
Brain Res. 2013 Sep 5;1529:39-45. doi: 10.1016/j.brainres.2013.07.030. Epub 2013 Jul 24.
2
Cometin is a novel neurotrophic factor that promotes neurite outgrowth and neuroblast migration in vitro and supports survival of spiral ganglion neurons in vivo.Cometin 是一种新型神经营养因子，能促进体外神经突生长和神经母细胞迁移，并支持体内螺旋神经节神经元的存活。
Exp Neurol. 2012 Jan;233(1):172-81. doi: 10.1016/j.expneurol.2011.09.027. Epub 2011 Oct 2.
3
Lithium alters the morphology of neurites regenerating from cultured adult spiral ganglion neurons.锂改变培养的成年耳蜗螺旋神经节神经元再生轴突的形态。
Hear Res. 2013 Oct;304:137-44. doi: 10.1016/j.heares.2013.07.001. Epub 2013 Jul 12.
4
Overexpression of Bcl-2 or Bcl-xL prevents spiral ganglion neuron death and inhibits neurite growth.Bcl-2或Bcl-xL的过表达可防止螺旋神经节神经元死亡并抑制神经突生长。
Dev Neurobiol. 2007 Feb 15;67(3):316-25. doi: 10.1002/dneu.20346.
5
Valproic acid promotes the neuronal differentiation of spiral ganglion neural stem cells with robust axonal growth.丙戊酸促进螺旋神经节神经干细胞的神经元分化，具有强大的轴突生长。
Biochem Biophys Res Commun. 2018 Sep 18;503(4):2728-2735. doi: 10.1016/j.bbrc.2018.08.032. Epub 2018 Aug 16.
6
[Effect of fibroblast growth factor-1 (FGF-1) on spiral ganglion cells of the mammalian cochlea].[成纤维细胞生长因子-1（FGF-1）对哺乳动物耳蜗螺旋神经节细胞的影响]
HNO. 2000 Jun;48(6):457-61. doi: 10.1007/s001060050597.
7
Members of the BMP, Shh, and FGF morphogen families promote chicken statoacoustic ganglion neurite outgrowth and neuron survival in vitro.BMP、Shh 和 FGF 形态发生素家族成员促进鸡前庭下神经节神经突的体外生长和神经元存活。
Dev Neurobiol. 2012 Sep;72(9):1213-28. doi: 10.1002/dneu.20988. Epub 2012 Jul 20.
8
Neuronal Survival, Morphology and Outgrowth of Spiral Ganglion Neurons Using a Defined Growth Factor Combination.使用特定生长因子组合的螺旋神经节神经元的神经元存活、形态和生长
PLoS One. 2015 Aug 11;10(8):e0133680. doi: 10.1371/journal.pone.0133680. eCollection 2015.
9
Survival, synaptogenesis, and regeneration of adult mouse spiral ganglion neurons in vitro.成年小鼠螺旋神经节神经元在体外的存活、突触形成及再生
Dev Neurobiol. 2007 Jan;67(1):108-22. doi: 10.1002/dneu.20336.
10
Influence of central glia on spiral ganglion neuron neurite growth.中枢神经胶质对螺旋神经节神经元轴突生长的影响。
Neuroscience. 2011 Mar 17;177:321-34. doi: 10.1016/j.neuroscience.2011.01.014. Epub 2011 Jan 14.
引用本文的文献
1
Fibroblast growth factor 8: Multifaceted role in development and developmental disorder.成纤维细胞生长因子8：在发育及发育障碍中的多方面作用
Genes Dis. 2025 Jan 10;12(5):101524. doi: 10.1016/j.gendis.2025.101524. eCollection 2025 Sep.
2
Decreasing the physical gap in the neural-electrode interface and related concepts to improve cochlear implant performance.缩小神经电极界面的物理间隙及相关概念以改善人工耳蜗性能。
Front Neurosci. 2024 Jul 24;18:1425226. doi: 10.3389/fnins.2024.1425226. eCollection 2024.
3
Tissue engineering strategies for spiral ganglion neuron protection and regeneration.用于螺旋神经节神经元保护和再生的组织工程策略
J Nanobiotechnology. 2024 Jul 31;22(1):458. doi: 10.1186/s12951-024-02742-8.
4
Fibroblast growth factor signaling in axons: from development to disease.成纤维细胞生长因子信号在轴突中的作用：从发育到疾病。
Cell Commun Signal. 2023 Oct 16;21(1):290. doi: 10.1186/s12964-023-01284-0.
5
Ca homeostasis maintained by TMCO1 underlies corpus callosum development via ERK signaling.TMCO1 通过 ERK 信号维持钙稳态，从而为胼胝体发育提供基础。
Cell Death Dis. 2022 Aug 4;13(8):674. doi: 10.1038/s41419-022-05131-x.
6
Regulation of Spiral Ganglion Neuron Regeneration as a Therapeutic Strategy in Sensorineural Hearing Loss.螺旋神经节神经元再生的调控作为感音神经性听力损失的一种治疗策略。
Front Mol Neurosci. 2022 Jan 20;14:829564. doi: 10.3389/fnmol.2021.829564. eCollection 2021.
7
Efficient induction of functional ameloblasts from human keratinocyte stem cells.高效诱导人角质细胞干细胞分化为功能性成釉细胞。
Stem Cell Res Ther. 2018 May 2;9(1):126. doi: 10.1186/s13287-018-0822-4.
8
Novel High Content Screen Detects Compounds That Promote Neurite Regeneration from Cochlear Spiral Ganglion Neurons.新型高内涵筛选检测出促进耳蜗螺旋神经节神经元轴突再生的化合物。
Sci Rep. 2015 Nov 2;5:15960. doi: 10.1038/srep15960.
9
Phosphodiesterase type 4 inhibitor rolipram improves survival of spiral ganglion neurons in vitro.磷酸二酯酶 4 抑制剂罗利普兰可改善体外耳蜗螺旋神经节神经元的存活。
PLoS One. 2014 Mar 18;9(3):e92157. doi: 10.1371/journal.pone.0092157. eCollection 2014.
本文引用的文献
1
FGF-2 low molecular weight selectively promotes neuritogenesis of motor neurons in vitro.成纤维细胞生长因子 2 低分子量选择性促进体外运动神经元的神经突生成。
Mol Neurobiol. 2013 Apr;47(2):770-81. doi: 10.1007/s12035-012-8389-z. Epub 2012 Dec 30.
2
Members of the BMP, Shh, and FGF morphogen families promote chicken statoacoustic ganglion neurite outgrowth and neuron survival in vitro.BMP、Shh 和 FGF 形态发生素家族成员促进鸡前庭下神经节神经突的体外生长和神经元存活。
Dev Neurobiol. 2012 Sep;72(9):1213-28. doi: 10.1002/dneu.20988. Epub 2012 Jul 20.
3
Multiple transcription factor families regulate axon growth and regeneration.多种转录因子家族调节轴突生长和再生。
Dev Neurobiol. 2011 Dec;71(12):1186-211. doi: 10.1002/dneu.20934.
4
Functional role of neurotrophin-3 in synapse regeneration by spiral ganglion neurons on inner hair cells after excitotoxic trauma in vitro.神经营养因子-3 在体外兴奋性中毒损伤后螺旋神经节神经元对内毛细胞突触再生的功能作用。
J Neurosci. 2011 May 25;31(21):7938-49. doi: 10.1523/JNEUROSCI.1434-10.2011.
5
Numerous isoforms of Fgf8 reflect its multiple roles in the developing brain.Fgf8 有许多同工型，反映了它在发育中的大脑中的多种作用。
J Cell Physiol. 2011 Jul;226(7):1722-6. doi: 10.1002/jcp.22587.
6
Increased expression of bFGF is associated with carotid atherosclerotic plaques instability engaging the NF-κB pathway.碱性成纤维细胞生长因子（bFGF）表达增加与通过核因子κB（NF-κB）途径参与的颈动脉粥样硬化斑块不稳定性相关。
J Cell Mol Med. 2010 Sep;14(9):2273-80. doi: 10.1111/j.1582-4934.2010.01082.x.
7
1-tert-butyl-3-[6-(3,5-dimethoxy-phenyl)-2-(4-diethylamino-butylamino)-pyrido[2,3-d]pyrimidin-7-yl]-urea (PD173074), a selective tyrosine kinase inhibitor of fibroblast growth factor receptor-3 (FGFR3), inhibits cell proliferation of bladder cancer carrying the FGFR3 gene mutation along with up-regulation of p27/Kip1 and G1/G0 arrest.1-叔丁基-3-[6-(3,5-二甲氧基苯基)-2-(4-二乙氨基丁基氨基)吡啶并[2,3-d]嘧啶-7-基]-脲（PD173074），一种成纤维细胞生长因子受体-3（FGFR3）的选择性酪氨酸激酶抑制剂，可抑制携带 FGFR3 基因突变的膀胱癌细胞的增殖，同时上调 p27/Kip1 并使细胞停滞于 G1/G0 期。
J Pharmacol Exp Ther. 2010 Mar;332(3):795-802. doi: 10.1124/jpet.109.162768. Epub 2009 Dec 2.
8
Age-related loss of spiral ganglion neurons.与年龄相关的螺旋神经节神经元丧失。
Hear Res. 2010 Jun 1;264(1-2):93-7. doi: 10.1016/j.heares.2009.10.009. Epub 2009 Oct 23.
9
The role of Fgf8 in telencephalic and diencephalic patterning.
Semin Cell Dev Biol. 2009 Aug;20(6):719-25. doi: 10.1016/j.semcdb.2009.04.002. Epub 2009 Apr 10.
10
Signaling mechanisms of neurite outgrowth induced by the cell adhesion molecules NCAM and N-cadherin.细胞粘附分子NCAM和N-钙粘蛋白诱导神经突生长的信号传导机制。
Cell Mol Life Sci. 2008 Nov;65(23):3809-21. doi: 10.1007/s00018-008-8290-0.
Zotero 插件