猪尾猕猴诱导多能干细胞来源的肝细胞支持丙型肝炎病毒感染。
Hepatic cells derived from induced pluripotent stem cells of pigtail macaques support hepatitis C virus infection.
机构信息
Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York.
出版信息
Gastroenterology. 2013 Nov;145(5):966-969.e7. doi: 10.1053/j.gastro.2013.07.026. Epub 2013 Jul 25.
Abstract
The narrow species tropism of hepatitis C virus (HCV) limits animal studies. We found that pigtail macaque (Macaca nemestrina) hepatic cells derived from induced pluripotent stem cells support the entire HCV life cycle, although infection efficiency was limited by defects in the HCV cell entry process. This block was overcome by either increasing occludin expression, complementing the cells with human CD81, or infecting them with a strain of HCV with less restricted requirements for CD81. Using this system, we can modify viral and host cell genetics to make pigtail macaques a suitable, clinically relevant model for the study of HCV infection.
摘要
丙型肝炎病毒 (HCV) 的狭窄种属趋向限制了动物研究。我们发现，来源于诱导多能干细胞的食蟹猴（Macaca nemestrina）肝细胞支持 HCV 的整个生命周期，尽管感染效率受到 HCV 细胞进入过程缺陷的限制。通过增加occludin 的表达、用人类 CD81 补充细胞，或者用对 CD81 要求较低的 HCV 株感染细胞，可以克服这种阻断。利用该系统，我们可以修饰病毒和宿主细胞的遗传学，使食蟹猴成为 HCV 感染研究的合适的、具有临床相关性的模型。
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