Computational Biology Center, Sloan-Kettering Institute for Cancer Research, New York, NY, USA.
FEBS Lett. 2013 Sep 2;587(17):2891-4. doi: 10.1016/j.febslet.2013.07.032. Epub 2013 Jul 23.
The Michaelis-Menten equation for an irreversible enzymatic reaction depends linearly on the enzyme concentration. Even if the enzyme concentration changes in time, this linearity implies that the amount of substrate depleted during a given time interval depends only on the average enzyme concentration. Here, we use a time re-scaling approach to generalize this result to a broad category of multi-reaction systems, whose constituent enzymes have the same dependence on time, e.g. they belong to the same regulon. This "average enzyme principle" provides a natural methodology for jointly studying metabolism and its regulation.
不可逆酶反应的米氏方程取决于酶浓度的线性关系。即使酶浓度随时间变化，这种线性关系意味着在给定时间间隔内消耗的底物量仅取决于平均酶浓度。在这里，我们使用时间重标方法将这一结果推广到一个广泛的多反应系统类别，其组成酶具有相同的时间依赖性，例如它们属于同一调控子。这种“平均酶原理”为共同研究代谢及其调控提供了一种自然的方法。
