Yamamoto Kota, Li Xin, Shu Chang, Miyata Tetsuro, Dardik Alan
Department of Surgery and the Interdepartmental Program in Vascular Biology and Therapeutics, Yale University, USA.
J Vis Exp. 2013 Jul 11(77):e50449. doi: 10.3791/50449.
Technical aspects of creating an arteriovenous fistula in the mouse are discussed. Under general anesthesia, an abdominal incision is made, and the aorta and inferior vena cava (IVC) are exposed. The proximal infrarenal aorta and the distal aorta are dissected for clamp placement and needle puncture, respectively. Special attention is paid to avoid dissection between the aorta and the IVC. After clamping the aorta, a 25 G needle is used to puncture both walls of the aorta into the IVC. The surrounding connective tissue is used for hemostatic compression. Successful creation of the AVF will show pulsatile arterial blood flow in the IVC. Further confirmation of successful AVF can be achieved by post-operative Doppler ultrasound.
本文讨论了在小鼠体内创建动静脉瘘的技术要点。在全身麻醉下，切开腹部，暴露主动脉和下腔静脉（IVC）。分别解剖肾下主动脉近端和远端主动脉，以便放置夹子和进行针刺。要特别注意避免在主动脉和IVC之间进行解剖。夹住主动脉后，用25G针头穿刺主动脉的两层壁进入IVC。使用周围的结缔组织进行止血压迫。成功创建动静脉瘘会显示IVC中有搏动性动脉血流。术后通过多普勒超声可进一步确认动静脉瘘是否成功。
