BioAnalytical Chemistry and Nanobiomedicine Laboratory, Department of Biochemical Science and Technology, National Taiwan University, Taipei 10617, Taiwan; Department of Chemistry, National Tsing Hua University, Hsinchu 30013, Taiwan.
Biosens Bioelectron. 2013 Dec 15;50:414-20. doi: 10.1016/j.bios.2013.06.044. Epub 2013 Jul 13.
Genetically modified (GM) technique, one of the modern biomolecular engineering technologies, has been deemed as profitable strategy to fight against global starvation. Yet rapid and reliable analytical method is deficient to evaluate the quality and potential risk of such resulting GM products. We herein present a biomolecular analytical system constructed with distinct biochemical activities to expedite the computational detection of genetically modified organisms (GMOs). The computational mechanism provides an alternative to the complex procedures commonly involved in the screening of GMOs. Given that the bioanalytical system is capable of processing promoter, coding and species genes, affirmative interpretations succeed to identify specified GM event in terms of both electrochemical and optical fashions. The biomolecular computational assay exhibits detection capability of genetically modified DNA below sub-nanomolar level and is found interference-free by abundant coexistence of non-GM DNA. This bioanalytical system, furthermore, sophisticates in array fashion operating multiplex screening against variable GM events. Such a biomolecular computational assay and biosensor holds great promise for rapid, cost-effective, and high-fidelity screening of GMO.
转基因技术是现代生物分子工程技术之一，被认为是应对全球饥饿的有利策略。然而，快速可靠的分析方法对于评估此类转基因产品的质量和潜在风险是不足的。本文构建了一个具有独特生化活性的生物分子分析系统，以加速对转基因生物（GMO）的计算检测。该计算机制为筛选 GMO 中常见的复杂程序提供了一种替代方法。鉴于该生物分析系统能够处理启动子、编码和物种基因，电化学和光学方法都可以成功地对特定的 GM 事件进行阳性解释。该生物分子计算测定法对亚纳摩尔以下的转基因 DNA 具有检测能力，并且由于大量共存的非 GM DNA 而不受干扰。此外，该生物分析系统以数组方式操作，可针对不同的 GM 事件进行多重筛选。这种生物分子计算测定法和生物传感器具有快速、经济高效、高保真筛选 GMO 的巨大潜力。
