Chen Hongxu, Tang Chun, Liu Menggang, Zhou Bo, Kuang Yi, Yuan Tao, Chen Ping
Department of Hepatobiliary Surgery, Daping Hospital, The Third Military Medical University, No. 10 Changjiangzhilu Daping, Chongqing, 400042, People's Republic of China.
Tumour Biol. 2014 Jan;35(1):27-32. doi: 10.1007/s13277-013-1001-y. Epub 2013 Jul 27.
The human X-ray repair cross-complementing group 1 gene (XRCC1) is an important candidate gene for affecting pancreatic cancer (PC) risk. The objective of this study was to detect whether the c.1471G > A and c.1686C > G polymorphisms of XRCC1 gene influence PC risk. The association of XRCC1 genetic variants with PC risk was analyzed in 328 PC patients and 350 controls by the polymerase chain reaction-restriction fragment length polymorphism and created restriction site-polymerase chain reaction method. Our data suggested that the genotypes and alleles from these two genetic variants were statistically associated with PC risk. For c.1471G > A, the AA genotype was associated with the decreased risk of developing PC compared to GG wild genotype (odds ratio (OR) = 0.43, 95% confidence intervals (CI) 0.26-0.70, chi-squared (χ(2)) = 11.91, P = 0.001). For c.1686C > G, the risk of PC was significantly lower for GG genotype in comparing to CC wild genotype (OR = 0.48, 95% CI 0.29-0.81, χ(2) = 7.98, P = 0.005). The A allele of c.1471G > A and G allele of c.1686C > G genetic variants could contribute to decrease the risk of PC (for c.1471G > A: A vs G, OR = 0.65, 95% CI 0.52-0.82, χ(2) = 13.71, P < 0.001, for c.1686C > G: G vs C, OR = 0.70, 95% CI 0.55-0.88, χ(2) = 9.42, P = 0.002). Our findings indicate that the c.1471G > A and c.1686C > G polymorphisms of XRCC1 gene are associated with PC risk in Chinese population.
人类X射线修复交叉互补基因1（XRCC1）是影响胰腺癌（PC）风险的重要候选基因。本研究的目的是检测XRCC1基因的c.1471G＞A和c.1686C＞G多态性是否影响PC风险。采用聚合酶链反应-限制性片段长度多态性和创造限制性位点-聚合酶链反应方法，分析了328例PC患者和350例对照中XRCC1基因变异与PC风险的关联。我们的数据表明，这两个基因变异的基因型和等位基因与PC风险在统计学上相关。对于c.1471G＞A，与GG野生基因型相比，AA基因型与患PC风险降低相关（优势比（OR）=0.43，95%置信区间（CI）0.26-0.70，卡方（χ(2)）=11.91，P=0.001）。对于c.1686C＞G，与CC野生基因型相比，GG基因型的PC风险显著降低（OR=0.48，95%CI 0.29-0.81，χ(2)=7.98，P=0.005）。c.1471G＞A的A等位基因和c.1686C＞G基因变异的G等位基因可能有助于降低PC风险（对于c.1471G＞A：A与G相比，OR=0.65，95%CI 0.52-0.82，χ(2)=13.71，P＜0.001，对于c.1686C＞G：G与C相比，OR=0.70，95%CI 0.55-0.88，χ(2)=9.42，P=0.002）。我们的研究结果表明，XRCC1基因的c.1471G＞A和c.1686C＞G多态性与中国人群的PC风险相关。
