Jin Zheng, Xu Haiyang, Zhang Xianfeng, Zhao Gang
Department of Neurosurgery, The First Bethune Hospital, Jilin University, No. 71 Xinmin Street, Changchun, 130021, Jilin Province, People's Republic of China.
Tumour Biol. 2014 Jan;35(1):357-62. doi: 10.1007/s13277-013-1050-2. Epub 2013 Aug 6.
Glioma is the most common type of primary brain malignancy in adults. The X-ray repair cross-complementing group 1 (XRCC1) is an important candidate gene for influencing the pathogenesis of glioma. This study aimed to evaluate the potential association between XRCC1 genetic polymorphisms and glioma susceptibility. This case-control study was conducted in Chinese Han populations consisting of 620 glioma cases and 630 cancer-free controls. XRCC1 genetic polymorphisms were detected by the polymerase chain reaction-restriction fragment length polymorphism and verified using DNA sequencing methods. The c.910A>G and c.1779C>G genetic polymorphisms were identified in this study. Our data suggested that the genotypes/alleles of these two genetic polymorphisms were statistically associated with the increased risk of glioma. As for c.910A>G, the risk of glioma for genotype GG was significantly higher than wild genotype AA (odds ratio (OR) = 1.98, 95% confidence interval (CI) 1.33-2.94, P = 0.001). As for c.1779C>G, the genotype GG was statistically associated with the increased risk of glioma compared to wild genotype CC (OR = 1.80, 95% CI 1.17-2.78, P = 0.007). Both of alleles G in c.910A>G and c.1779C>G may contribute to glioma susceptibility (G versus (vs.) A, OR = 1.30, 95% CI 1.09-1.54, P = 0.003; G vs. C, OR = 1.19, 95% CI 1.00-1.42, P = 0.045). Our findings indicate that the c.910A>G and c.1779C>G genetic polymorphisms are associated with the susceptibility to glioma in Chinese Han populations and might be used as molecular markers for evaluating glioma risk.
胶质瘤是成人中最常见的原发性脑恶性肿瘤类型。X射线修复交叉互补基因1（XRCC1）是影响胶质瘤发病机制的重要候选基因。本研究旨在评估XRCC1基因多态性与胶质瘤易感性之间的潜在关联。这项病例对照研究在中国汉族人群中进行，包括620例胶质瘤病例和630例无癌对照。采用聚合酶链反应-限制性片段长度多态性方法检测XRCC1基因多态性，并使用DNA测序方法进行验证。本研究鉴定出了c.910A>G和c.1779C>G基因多态性。我们的数据表明，这两种基因多态性的基因型/等位基因与胶质瘤风险增加在统计学上相关。对于c.910A>G，基因型GG患胶质瘤的风险显著高于野生基因型AA（比值比（OR）=1.98，95%置信区间（CI）1.33-2.94，P=0.001）。对于c.1779C>G，与野生基因型CC相比，基因型GG与胶质瘤风险增加在统计学上相关（OR=1.80，95%CI 1.17-2.78，P=0.007）。c.910A>G和c.1779C>G中的等位基因G均可能导致胶质瘤易感性（G与A相比，OR=1.30，95%CI 1.09-1.54，P=0.003；G与C相比，OR=1.19，95%CI 1.00-1.42，P=0.045）。我们的研究结果表明，c.910A>G和c.1779C>G基因多态性与中国汉族人群的胶质瘤易感性相关，可能用作评估胶质瘤风险的分子标志物。
