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本文引用的文献

1
Phosphorylation of centromeric histone H3 variant regulates chromosome segregation in Saccharomyces cerevisiae.着丝粒组蛋白 H3 变体的磷酸化调控酿酒酵母中的染色体分离。
Mol Biol Cell. 2013 Jun;24(12):2034-44. doi: 10.1091/mbc.E12-12-0893. Epub 2013 May 1.
2
A novel role of the N terminus of budding yeast histone H3 variant Cse4 in ubiquitin-mediated proteolysis.酿酒酵母组蛋白 H3 变体 Cse4 N 端在泛素介导的蛋白水解中的新作用。
Genetics. 2013 Jun;194(2):513-8. doi: 10.1534/genetics.113.149898. Epub 2013 Mar 22.
3
Exome and whole-genome sequencing of esophageal adenocarcinoma identifies recurrent driver events and mutational complexity.食管腺癌的外显子组和全基因组测序鉴定出反复出现的驱动事件和突变复杂性。
Nat Genet. 2013 May;45(5):478-86. doi: 10.1038/ng.2591. Epub 2013 Mar 24.
4
Genome and transcriptome sequencing of lung cancers reveal diverse mutational and splicing events.基因组和转录组测序揭示了肺癌中多样的突变和剪接事件。
Genome Res. 2012 Dec;22(12):2315-27. doi: 10.1101/gr.140988.112. Epub 2012 Oct 2.
5
NIH Image to ImageJ: 25 years of image analysis.NIH 图像到 ImageJ：25 年的图像分析。
Nat Methods. 2012 Jul;9(7):671-5. doi: 10.1038/nmeth.2089.
6
Interactions between the kinetochore complex and the protein kinase A pathway in Saccharomyces cerevisiae.酿酒酵母中着丝粒复合物与蛋白激酶 A 途径的相互作用。
G3 (Bethesda). 2012 Jul;2(7):831-41. doi: 10.1534/g3.112.002675. Epub 2012 Jul 1.
7
Cell-cycle-coupled structural oscillation of centromeric nucleosomes in yeast.酵母着丝粒核小体的细胞周期偶联结构振荡。
Cell. 2012 Jul 20;150(2):304-16. doi: 10.1016/j.cell.2012.05.034.
8
mRNA BRAF expression helps to identify papillary thyroid carcinomas in thyroid nodules independently of the presence of BRAFV600E mutation.mRNA BRAF 表达有助于在甲状腺结节中独立于 BRAFV600E 突变的存在来识别甲状腺乳头状癌。
Pathol Res Pract. 2012 Aug 15;208(8):489-92. doi: 10.1016/j.prp.2012.05.013. Epub 2012 Jul 5.
9
Mitotic spindle form and function.有丝分裂纺锤体的形成和功能。
Genetics. 2012 Apr;190(4):1197-224. doi: 10.1534/genetics.111.128710.
10
Flexibility of centromere and kinetochore structures.着丝粒和动粒结构的灵活性。
Trends Genet. 2012 May;28(5):204-12. doi: 10.1016/j.tig.2012.02.003. Epub 2012 Mar 23.

着丝粒染色质的结构完整性和忠实的染色体分离需要 Pat1。

Structural integrity of centromeric chromatin and faithful chromosome segregation requires Pat1.

机构信息

Genetics Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892.

出版信息

Genetics. 2013 Oct;195(2):369-79. doi: 10.1534/genetics.113.155291. Epub 2013 Jul 26.

DOI:10.1534/genetics.113.155291
PMID:23893485
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3781966/
Abstract

The kinetochore (centromeric DNA and associated protein complex) is essential for faithful chromosome segregation and maintenance of genome stability. Here we report that an evolutionarily conserved protein Pat1 is a structural component of Saccharomyces cerevisiae kinetochore and associates with centromeres in a NDC10-dependent manner. Consistent with a role for Pat1 in kinetochore structure and function, a deletion of PAT1 results in delay in sister chromatid separation, errors in chromosome segregation, and defects in structural integrity of centromeric chromatin. Pat1 is involved in topological regulation of minichromosomes as altered patterns of DNA supercoiling were observed in pat1Δ cells. Studies with pat1 alleles uncovered an evolutionarily conserved region within the central domain of Pat1 that is required for its association with centromeres, sister chromatid separation, and faithful chromosome segregation. Taken together, our data have uncovered a novel role for Pat1 in maintaining the structural integrity of centromeric chromatin to facilitate faithful chromosome segregation and proper kinetochore function.

摘要

着丝粒（着丝粒 DNA 和相关蛋白复合物）对于忠实的染色体分离和基因组稳定性的维持至关重要。在这里，我们报告说，一种进化上保守的蛋白质 Pat1 是酿酒酵母着丝粒的结构组成部分，并以 NDC10 依赖性的方式与着丝粒结合。与 Pat1 在着丝粒结构和功能中的作用一致，PAT1 的缺失导致姐妹染色单体分离延迟、染色体分离错误以及着丝粒染色质结构完整性缺陷。Pat1 参与了小型染色体的拓扑调控，因为在 pat1Δ 细胞中观察到 DNA 超螺旋模式的改变。对 pat1 等位基因的研究揭示了 Pat1 中心结构域内的一个进化上保守区域，该区域对于其与着丝粒、姐妹染色单体分离和忠实染色体分离的关联是必需的。总之，我们的数据揭示了 Pat1 在维持着丝粒染色质结构完整性以促进忠实染色体分离和适当的着丝粒功能方面的新作用。