Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, Kansas; Institute of Reproductive Health and Regenerative Medicine, University of Kansas Medical Center, Kansas City, Kansas.
Mol Reprod Dev. 2014 Feb;81(2):171-82. doi: 10.1002/mrd.22219. Epub 2013 Aug 13.
The first cell-fate decision in mammalian preimplantation embryos is the segregation of the inner cell mass (ICM) and trophectoderm (TE) cell lineages. The ICM develops into the embryo proper, whereas the TE ensures embryo implantation and is the source of the extra-embryonic trophoblast cell lineages, which contribute to the functional components of the placenta. The development of a totipotent zygote into a multi-lineage blastocyst is associated with the generation of distinct transcriptional programs. Several key transcription factors participate in the ICM and TE-specific transcriptional networks, and recent studies indicate that post-translational histone modifications as well as ATP-dependent chromatin remodeling complexes converge with these transcriptional networks to regulate ICM and TE lineage specification. This review will discuss our current understanding and future perspectives related to transcriptional and epigenetic regulatory mechanisms that are implicated in the initial mammalian lineage commitment steps, with a focus on events in mice.
哺乳动物着床前胚胎的第一个细胞命运决定是内细胞团（ICM）和滋养外胚层（TE）细胞谱系的分离。ICM 发育成胚胎本身，而 TE 确保胚胎着床，并且是胚胎外滋养层细胞谱系的来源，这些细胞谱系有助于胎盘的功能成分。全能合子发育成多能胚泡与独特的转录程序的产生有关。几个关键的转录因子参与 ICM 和 TE 特异性转录网络，最近的研究表明，翻译后组蛋白修饰以及 ATP 依赖性染色质重塑复合物与这些转录网络汇聚，以调节 ICM 和 TE 谱系的特化。这篇综述将讨论我们目前对与初始哺乳动物谱系决定步骤相关的转录和表观遗传调控机制的理解和未来展望，重点是在小鼠中的事件。
