Augot D, Randrianambinintsoa F J, Gasser A, Depaquit J
ANSES-LSA, Université Champagne-Ardenne, Faculté de Pharmacie, EA4688, SFR Cap Santé, Usc Transmission Vectorielle et épidemiosurveillance de maladies Parasitaires (VECPAR), Reims, France.
Bull Soc Pathol Exot. 2013 Aug;106(3):201-5. doi: 10.1007/s13149-013-0302-4. Epub 2013 Jul 20.
Culicoides are vectors of diseases of Veterinary Medicine importance (bluetongue, African horse sickness, Schmallenberg virus) all over the world. In the present study, we report two species new for Madagascar: C. nevilli and C. enderleini. They belong to the Schultzei group which is sometimes classified in the subgenus Oecacta and sometimes in the subgenus Remmia, depending on authors. Consequently, we carried out a molecular cladistics of these groups based on cytochrome C oxidase subunit I mtDNA sequences. We processed the Malagasy specimens and some C. furens (the Oecacta type-species) caught in Florida and we analyzed their sequences and those available in Genbank: C. schultzei, C. oxystoma, C. festivipennis, C. brunnicans, C. kibunensis, C. truncorum and C. vexans. C. (Avaritia) imicola have been selected as an outgroup. The maximum parsimony analysis showed the paraphylies of the Schultzei group (=Remmia) and of the subgenus Oecacta if the first group is excluded from the latter. Our results underline the doubtful current classification and need to be validated by other molecular markers in the future.
库蠓是世界各地兽医医学重要疾病（蓝舌病、非洲马瘟、施马伦贝格病毒）的传播媒介。在本研究中，我们报告了马达加斯加两种新发现的库蠓物种：内维利库蠓和恩德勒伊尼古蠓。它们属于舒尔策伊组，根据不同作者的分类，该组有时被归入奥埃卡塔亚属，有时被归入雷米亚亚属。因此，我们基于细胞色素C氧化酶亚基I线粒体DNA序列对这些类群进行了分子系统发育分析。我们对马达加斯加的标本以及在佛罗里达州捕获的一些富伦库蠓（奥埃卡塔亚属的模式种）进行了处理，并分析了它们的序列以及基因库中可用的序列：舒尔策伊库蠓、尖口库蠓、华丽库蠓、褐足库蠓、基布纳库蠓、截尾库蠓和骚扰库蠓。已选择伊米科拉库蠓（阿瓦里蒂亚亚属）作为外类群。最大简约法分析表明，如果将舒尔策伊组（=雷米亚亚属）排除在奥埃卡塔亚属之外，那么舒尔策伊组和奥埃卡塔亚属都具有并系性。我们的结果强调了当前分类的可疑性，未来需要通过其他分子标记进行验证。
