三种酶联免疫吸附测定试剂盒检测肝片吸虫病的效果评估

[Effect evaluation of three ELISA kits in detection of fasciolasis].

作者信息

Ai Lin, Chen Mu-Xin, Chen Shao-Hong, Chu Yan-Hong, Cai Yu-Chun, Zhou Xiao-Nong, Chen Jia-Xu

机构信息

National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, WHO Collaborating Center of Malaria, Schistosomiasis and Filariasis, Shanghai 200025, China

出版信息

Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2013 Apr;25(2):177-81.

PMID:23894840

Abstract

OBJECTIVE

To evaluate the effect of 3 ELISA kits on detection of human fasciolasis.

METHODS

Twenty-six serum samples from patients with fasciolasis, 180 serum samples from patients with other parasitic diseases as well as 26 serum samples from healthy people were detected by ELISA kits which using soluble antigen of Fasciola gigantica, Fasciola hepatica (Fg-ELISA and Fh-ELISA) as well as IgG antigen ELISA detection kits made by DRG company in Germany. The effects of the 3 kits were evaluated.

RESULTS

The sensitivities of Fg-ELISA, Fh-ELISA, and DRG-ELISA were 100.0%, 80.8% (95% CI: 65.7%-95.9%) and 100.0%, respectively; the specificities of the three were 87.9% (95% CI: 83.5%-92.4%), 85.0%(95% CI: 80.1%-89.9%) and 83.5% (95% CI: 78.4%-88.6%), respectively, and Youden indexes of them were 0.88, 0.66 and 0.84, respectively. The detection rate of Fg-ELISA (100%) was significantly higher than that of Fh-ELISA (80.8%) (P < 0.05). The A absolute value (A/CO) of Fg-ELISA was 1.70, which was also significantly higher than the value of Fh-ELISA (1.18) (P < 0.000 1).

CONCLUSION

Fg-ELISA has a good detection effect and low cost, and is more suitable than Fh-ELISA and DRG-ELISA for clinical sample tests as well as massive screening in fasciolasis endemic areas in southwest China.

摘要

目的

评估3种酶联免疫吸附测定（ELISA）试剂盒检测人体肝片吸虫病的效果。

方法

采用以大片吸虫、肝片吸虫可溶性抗原（Fg-ELISA和Fh-ELISA）以及德国DRG公司生产的IgG抗原ELISA检测试剂盒，对26份肝片吸虫病患者血清样本、180份其他寄生虫病患者血清样本以及26份健康人血清样本进行检测，评估3种试剂盒的检测效果。

结果

Fg-ELISA、Fh-ELISA和DRG-ELISA的灵敏度分别为100.0%、80.8%（95%可信区间：65.7% - 95.9%）和100.0%；三者的特异性分别为87.9%（95%可信区间：83.5% - 92.4%）、85.0%（95%可信区间：80.1% - 89.9%）和83.5%（95%可信区间：78.4% - 88.6%），其约登指数分别为0.88、0.66和0.84。Fg-ELISA的检出率（100%）显著高于Fh-ELISA（80.8%）（P < 0.05）。Fg-ELISA的A绝对值（A/CO）为1.70，也显著高于Fh-ELISA的值（1.18）（P < 0.000 1）。