Department of Life and Reproduction Sciences, University of Verona, Verona, Italy.
Biochem Med (Zagreb). 2013;23(2):201-5. doi: 10.11613/bm.2013.023.
It is still uncertain whether or not avoidance to let disinfectant alcohol dry at the site of venipuncture is a source of spurious hemolysis when drawing venous blood.
In a consecutive series of 52 outpatients referred for routine laboratory testing, venous blood was drawn by direct venipuncture with (odd group) or without (pair group) wiping 70% isopropyl alcohol at the site of venipuncture. A 3.5 mL evacuated tube with dot activator and gel separator was drawn from a vein of the upper limb, serum was immediately separated with standard centrifugation and tested for potassium, lactate dehydrogenase (LD), aspartate aminotransferase (AST) and hemolysis index (HI) on Roche Cobas.
No specimen was discarded for unsatisfactory venipuncture. No differences for age and gender were observed between groups. As regards the four parameters investigated, no significant differences could be observed between patients in whom blood was drawn with or without letting the alcohol dry. It is also noteworthy that no sample in both groups exceeded the conventional sample rejection threshold of cell-free hemoglobin.
The results of our prospective, randomized study attest that failure to wipe alcohol at the site of venipuncture should not be considered as a potential source of spurious hemolysis when drawing blood.
在采集静脉血时，避免让消毒剂酒精在穿刺部位干燥是否会导致假性溶血，目前仍不确定。
在连续的 52 例门诊患者中，通过直接静脉穿刺采集静脉血，一组（奇数组）在穿刺部位擦拭 70%异丙醇，另一组（偶数组）不擦拭。从上肢静脉抽取 3.5 mL 带点状激活剂和凝胶分离剂的真空采血管，用标准离心法立即分离血清，并在罗氏 Cobas 上检测钾、乳酸脱氢酶（LD）、天门冬氨酸氨基转移酶（AST）和溶血指数（HI）。
没有因静脉穿刺不满意而丢弃的标本。两组患者在年龄和性别方面无差异。对于所研究的四个参数，在擦拭酒精和不擦拭酒精的患者之间未观察到显著差异。值得注意的是，两组均无样本超过无细胞血红蛋白的常规样本拒收阈值。
本前瞻性、随机研究的结果表明，在采血时，未能擦拭穿刺部位的酒精不应被视为假性溶血的潜在来源。
