[宫颈癌组织中La蛋白的表达及其临床意义]
[La protein expression in cervical cancer tissues and its clinical significance].
作者信息
Chen Kunlun, Wu Yi, Li Mu, Li Lan, Gao Yane, Gao Qing
机构信息
Xi'an Jiaotong University College of Medicine, Xi'an, China.
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2013 Jul;33(7):999-1003.
OBJECTIVE
To investigate the expression of La protein in cervical cancer tissues and explore its role in the occurrence and progression of cervical cancer.
METHODS
The expression of La protein in cervical cancer and normal cervical tissues was detected by immunohistochemical staining. RNA interference technology was used to silence La protein expression in HeLa cells and the changes in cell proliferation, tumor sphere formation and cell cycles were investigated.
RESULTS
The expression of La protein was significantly higher in cervical cancer tissues than in normal cervical tissues (61% vs 9%, P<0.05). Silencing La protein expression in HeLa cells caused significantly reduced the cell proliferation and lowered the tumor sphere formation rate from the control level of (17.1=1.92)% to (6.3=0.45)% (P<0.05), resulting also in G0/G1 cell cycle arrest and reduced cyclin D1 protein expression.
CONCLUSION
The RNA binding protein La can promote the development of cervical cancer and may play a critical role in the carcinogenesis and progression of cervical cancer.
目的
研究La蛋白在宫颈癌组织中的表达情况，并探讨其在宫颈癌发生发展中的作用。
方法
采用免疫组织化学染色法检测宫颈癌组织和正常宫颈组织中La蛋白的表达。运用RNA干扰技术沉默HeLa细胞中La蛋白的表达，研究细胞增殖、肿瘤球形成及细胞周期的变化。
结果
宫颈癌组织中La蛋白的表达显著高于正常宫颈组织（61%对9%，P<0.05）。沉默HeLa细胞中La蛋白的表达可显著降低细胞增殖，使肿瘤球形成率从对照水平的（17.1±1.92）%降至（6.3±0.45）%（P<0.05），还导致G0/G1期细胞周期阻滞并降低细胞周期蛋白D1的蛋白表达。
结论
RNA结合蛋白La可促进宫颈癌的发展，可能在宫颈癌的发生和进展中起关键作用。
Zotero 插件