School of Biological Sciences, University of Reading, Reading, RG6 6UB, UK.
J Inorg Biochem. 2013 Nov;128:245-9. doi: 10.1016/j.jinorgbio.2013.07.004. Epub 2013 Jul 12.
Aluminium (Al) has been measured in human breast tissue, nipple aspirate fluid and breast cyst fluid, and recent studies have shown that at tissue concentrations, aluminium can induce DNA damage and suspension growth in human breast epithelial cells. This paper demonstrates for the first time that exposure to aluminium can also increase migratory and invasive properties of MCF-7 human breast cancer cells. Long-term (32 weeks) but not short-term (1 week) exposure of MCF-7 cells to 10(-4) M aluminium chloride or 10(-4) M aluminium chlorohydrate increased motility of the cells as measured by live cell imaging (cumulative length moved by individual cells), by a wound healing assay and by migration in real time through 8 μm pores of a membrane using xCELLigence technology. Long-term exposure (37 weeks) to 10(-4) M aluminium chloride or 10(-4) M aluminium chlorohydrate also increased the ability of MCF-7 cells to invade through a matrigel layer as measured in real time using the xCELLigence system. Although molecular mechanisms remain to be characterized, the ability of aluminium salts to increase migratory and invasive properties of MCF-7 cells suggests that the presence of aluminium in the human breast could influence metastatic processes. This is important because mortality from breast cancer arises mainly from tumour spread rather than from the presence of a primary tumour in the breast.
铝（Al）已在人乳组织、乳头吸出液和乳腺囊肿液中进行了测量，最近的研究表明，在组织浓度下，铝可诱导人乳腺上皮细胞中的 DNA 损伤和悬浮生长。本文首次证明，暴露于铝还可以增加 MCF-7 人乳腺癌细胞的迁移和侵袭特性。长期（32 周）而非短期（1 周）暴露于 10(-4) M 氯化铝或 10(-4) M 碱式氯化铝可增加 MCF-7 细胞的运动性，这通过活细胞成像（单个细胞移动的累积长度）、划痕愈合测定和通过 xCELLigence 技术实时穿过膜上 8 μm 孔的迁移来测量。长期（37 周）暴露于 10(-4) M 氯化铝或 10(-4) M 碱式氯化铝也增加了 MCF-7 细胞穿过基质胶层的侵袭能力，这通过 xCELLigence 系统实时测量。尽管分子机制仍有待表征，但铝盐增加 MCF-7 细胞迁移和侵袭特性的能力表明，铝在人乳中的存在可能会影响转移过程。这很重要，因为乳腺癌的死亡率主要来自肿瘤扩散，而不是乳房中存在原发性肿瘤。
