Department of Chemistry and Food Toxicology, Institute of Food Technology and Nutrition, University of Rzeszów, Ćwiklinskiej 1a, 35-601 Rzeszow, Poland.
Department of Biotechnology and Bioinformatics, Faculty of Chemistry, Rzeszow University of Technology, Powstańców Warszawy 6, 35-959 Rzeszow, Poland.
Molecules. 2022 Jan 22;27(3):725. doi: 10.3390/molecules27030725.
Nine samples of ethanolic extracts of poplar-type propolis (EEP) originated from South-Eastern Poland were analyzed in terms of the diversity of the flora around the apiary. The mineral composition, antioxidant properties, polyphenolic profile (HPTLC), and main polyphenolic constituents (HPLC-DAD) were determined. Only minor differences in chemical composition and antioxidant capacity between tested EEPs were found regardless of their botanical origin. However, the biological activity of the EEPs was more diversified. The tested EEPs showed stronger antibacterial activity against Gram-negative bacteria () compared to Gram-positive bacteria ( and ). Staphylococci biofilm inhibition occurred as a result of exposure to the action of four out of nine EEPs (P1-P4). Due to the various compositions of individual EEPs, a different MCF-7 cellular response was observed according to inhibition of cells migration and proliferation. Almost every sample inhibited the migration of breast cancer cells at a low concentration (0.04 µg/mL) of propolis. Even at the lowest concentration (0.02 µg/mL), each EEP inhibited the proliferation of MCF-7 cells, however, the level of inhibition varied between samples.
九种源自波兰东南部的白杨型蜂胶（EEP）的乙醇提取物样品，从养蜂场周围的植物多样性方面进行了分析。测定了它们的矿物组成、抗氧化性能、多酚图谱（HPTLC）和主要多酚成分（HPLC-DAD）。无论其植物来源如何，测试的 EEPs 在化学成分和抗氧化能力方面只有很小的差异。然而，EEPs 的生物活性更加多样化。测试的 EEPs 对革兰氏阴性菌（）的抗菌活性明显强于革兰氏阳性菌（和）。四种 EEPs（P1-P4）的作用导致了葡萄球菌生物膜的抑制。由于各个 EEPs 的组成不同，根据对细胞迁移和增殖的抑制作用，观察到 MCF-7 细胞的不同反应。几乎每个样品都在低浓度（0.04μg/mL）的蜂胶下抑制乳腺癌细胞的迁移。即使在最低浓度（0.02μg/mL）下，每个 EEP 都抑制 MCF-7 细胞的增殖，但抑制水平在样品之间有所不同。
