Departamento de Neurorrehabilitación, Instituto Nacional de Rehabilitación, SSA, Mexico.
Neurosci Lett. 2013 Sep 27;552:5-9. doi: 10.1016/j.neulet.2013.07.026. Epub 2013 Jul 26.
Histamine H3 receptors (H3Rs) co-localize with dopamine (DA) D1 receptors (D1Rs) on striatal medium spiny neurons and functionally antagonize D1R-mediated responses. The intra-striatal administration of D1R agonists reduces DA release whereas D1R antagonists have the opposite effect. In this work, a microdialysis method was used to study the effect of co-activating D1 and H3 receptors on the release of DA from the rat dorsal striatum. Infusion of the D1R agonist SKF-38393 (0.5 and 1 μM) significantly reduced DA release (26-58%), and this effect was prevented by co-administration of the H3R agonist immepip (10 μM). In turn, the effect of immepip was blocked by the H3R antagonist thioperamide (10 μM). Our results indicate that co-stimulation of post-synaptic D1 and H3 receptors may indirectly regulate basal DA release in the rat striatum and provide in vivo evidence for a functional interaction between D1 and H3 receptors in the basal ganglia.
组胺 H3 受体 (H3R) 与纹状体中间多棘神经元上的多巴胺 (DA) D1 受体 (D1R) 共定位，并在功能上拮抗 D1R 介导的反应。纹状体内给予 D1R 激动剂可减少 DA 释放，而 D1R 拮抗剂则有相反的作用。在这项工作中，使用微透析方法研究了共激活 D1 和 H3 受体对大鼠背侧纹状体 DA 释放的影响。D1R 激动剂 SKF-38393（0.5 和 1 μM）的输注显著减少了 DA 的释放（26-58%），而 H3R 激动剂 immepip（10 μM）的共同给予则阻止了这种作用。反过来，immepip 的作用被 H3R 拮抗剂噻庚啶（10 μM）阻断。我们的结果表明，突触后 D1 和 H3 受体的共刺激可能间接调节大鼠纹状体中的基础 DA 释放，并为基底神经节中 D1 和 H3 受体之间的功能相互作用提供了体内证据。
