体外细胞遗传学检测：染色体畸变和微核试验。

In vitro cytogenetic assays: chromosomal aberrations and micronucleus tests.

作者信息

Mosesso Pasquale, Cinelli Serena, Natarajan Adyapalam T, Palitti Fabrizio

机构信息

Dipartimento di Scienze Ecologiche e Biologiche, Università degli Studi della Tuscia, Largo dell'Università, Viterbo, Italy.

出版信息

Methods Mol Biol. 2013;1044:123-46. doi: 10.1007/978-1-62703-529-3_6.

DOI:10.1007/978-1-62703-529-3_6

PMID:23896874

Abstract

Chromosome damage is a very important indicator of genetic damage relevant to environmental and clinical studies. Detailed descriptions of the protocols used for detection of chromosomal aberrations induced by unknown agents in vitro both in the presence or the absence of rat liver-derived metabolizing systems are given. Structural chromosomal aberrations that can be observed and quantified at metaphases are described here. For the detection of chromosomal damage (fragments or whole chromosome) in interphase, the micronucleus test can be used and a description of this test is also presented. Criteria for determining a positive result using appropriate statistical methods are described.

摘要

染色体损伤是与环境和临床研究相关的遗传损伤的一个非常重要的指标。本文详细描述了在有或无大鼠肝脏来源的代谢系统的情况下，用于检测体外未知试剂诱导的染色体畸变的实验方案。这里描述了在中期可以观察和量化的结构性染色体畸变。为了检测间期的染色体损伤（片段或整条染色体），可以使用微核试验，本文也对该试验进行了描述。还描述了使用适当统计方法确定阳性结果的标准。