Xin Lili, Lin Yao, Wang Aiqing, Zhu Wei, Liang Yu, Su Xiaojun, Hong Chengjiao, Wan Jianmei, Wang Yarong, Tian Hailin
School of Public Health, Medical College of Soochow University, 199 Renai Road, Suzhou 215123, Jiangsu, China; Jiangsu Key Laboratory of Preventive and Translational Medicine for Geriatric Diseases, School of Public Health, Soochow University, Suzhou 215123, China.
Experimental Center of Medical College, Soochow University, 199 Renai Road, Suzhou 215123, Jiangsu, China.
Environ Toxicol Pharmacol. 2015 Sep;40(2):524-9. doi: 10.1016/j.etap.2015.08.002. Epub 2015 Aug 7.
Bisphenol A (BPA), identified as an endocrine disruptor, is an important man-made compound used in a wide range of consumer products. The MTT assay, comet assay, micronucleus test, chromosome aberration test, and Ames assay were conducted to assess the cytotoxic, genotoxic, cytogenetic effects, and mutagenic activity of BPA. After BPA exposure, we showed significant increases in cytotoxicity and level of DNA damage indicated by Olive tail moment, tail length, and % tail DNA in a similar dose- and time-dependent manner. Significant increases in micronucleus frequency and conventional chromosome aberrations were also observed after BPA treatment. The major types of structural aberrations were breaks, gaps, and fragments. However, no positive mutagenic activity of BPA was observed in any of the tester strains. Taken together, the data obtained in this study clearly demonstrated that BPA is not mutagenic but could exhibit significant genotoxic and cytogenetic effects in Chinese hamster ovary cells.
双酚A（BPA）被认定为一种内分泌干扰物，是一种广泛用于各类消费品的重要人造化合物。进行了MTT试验、彗星试验、微核试验、染色体畸变试验和艾姆斯试验，以评估双酚A的细胞毒性、遗传毒性、细胞遗传学效应和诱变活性。双酚A暴露后，我们发现细胞毒性以及由橄榄尾矩、尾长和尾DNA百分比所表明的DNA损伤水平以类似的剂量和时间依赖性方式显著增加。双酚A处理后还观察到微核频率和传统染色体畸变显著增加。结构畸变的主要类型为断裂、裂隙和片段。然而，在任何测试菌株中均未观察到双酚A的阳性诱变活性。综上所述，本研究获得的数据清楚地表明，双酚A不具有诱变作用，但在中国仓鼠卵巢细胞中可表现出显著的遗传毒性和细胞遗传学效应。
