体外细胞遗传学分析：染色体畸变和微核试验。

In Vitro Cytogenetic Assays: Chromosomal Aberrations and Micronucleus Tests.

作者信息

Mosesso Pasquale, Cinelli Serena

机构信息

Dipartimento di Scienze Ecologiche e Biologiche, Università degli Studi della Tuscia, Largo dell'Università, Viterbo, Italy.

Research Toxicology Centre S.p.A., Pomezia (Roma), Italy.

出版信息

Methods Mol Biol. 2019;2031:79-104. doi: 10.1007/978-1-4939-9646-9_4.

DOI:10.1007/978-1-4939-9646-9_4

PMID:31473955

Abstract

Chromosome damage is a very important indicator of genetic damage relevant to environmental and clinical studies. Detailed descriptions of the protocols used for detection of chromosomal aberrations induced by genotoxic agents in vitro both in the presence or absence of rat liver-derived metabolizing systems are given in this chapter. Structural chromosomal aberrations that can be observed and quantified at metaphases are described here. For the detection of chromosomal damage (fragments or whole chromosome) in interphase, the micronucleus test can be used, and a description of this test is also presented. Criteria for determining a positive result using appropriate statistical methods are described.

摘要

染色体损伤是与环境和临床研究相关的遗传损伤的一个非常重要的指标。本章详细描述了在有或没有大鼠肝脏来源的代谢系统的情况下，用于检测体外遗传毒性剂诱导的染色体畸变的实验方案。这里描述了在中期可以观察和定量的结构性染色体畸变。为了检测间期的染色体损伤（片段或整条染色体），可以使用微核试验，本章也对该试验进行了描述。还描述了使用适当统计方法确定阳性结果的标准。