Beijing, People's Republic of China From the Breast Plastic and Reconstructive Surgery Center, Plastic Surgery Hospital, Plastic Surgery Institute, and the Department of Pathology, Chinese Academy of Medical Sciences, Peking Union Medical College.
Plast Reconstr Surg. 2013 Aug;132(2):363-373. doi: 10.1097/PRS.0b013e31829588b3.
Some studies have suggested that adipose-derived stromal vascular fraction is a potential cell source responsible for the improved quality and long-term retention of fat grafts, but studies that have clearly demonstrated the survival and differentiation potential of the implanted stromal vascular fraction cells as being dynamic phenomena have not been widely reported.
The authors isolated stromal vascular fraction cells from C57BL/6J-GFP mice. Green fluorescence protein-positive stromal vascular fraction cells were mixed with minced inguinal adipose tissue harvested from C57BL/6J mice and then co-implanted into BALB/c nude mice. The survival of implanted green fluorescence protein-positive stromal vascular fraction cells was tracked by in vivo fluorescence imaging for 56 days. Immunofluorescence staining was used to analyze the differentiation of green fluorescence protein-positive stromal vascular fraction cells occurring in ischemic adipose tissue at 7, 14, 28, 35, 42, or 56 days.
The fluorescence signal intensity fell drastically within the first 14 days after co-implantation and continued to decrease thereafter, with 17.3 percent signal intensity (relative to day 1) at 56 days. Immunofluorescence staining revealed that some green fluorescence protein-positive cells can spontaneously differentiate into adipocytes from day 7, and some of the implanted stromal vascular fraction cells can incorporate into new blood vessels.
The authors show convincing evidence for dynamic changes of stromal vascular fraction cells after co-implantation with fat grafts. The results prove the principle that implanted stromal vascular fraction cells can survive in the ischemic microenvironment of fat grafts and participate in the process of adipogenesis and angiogenesis.
一些研究表明，脂肪来源的基质血管成分是一种潜在的细胞来源，可改善脂肪移植物的质量和长期保留，但明确表明植入的基质血管成分细胞的存活和分化潜力是动态现象的研究尚未广泛报道。
作者从 C57BL/6J-GFP 小鼠中分离基质血管成分细胞。绿色荧光蛋白阳性的基质血管成分细胞与从小鼠腹股沟获取的切碎的脂肪组织混合，然后共同植入 BALB/c 裸鼠。通过体内荧光成像在 56 天内追踪植入的绿色荧光蛋白阳性基质血管成分细胞的存活情况。免疫荧光染色分析了在缺血性脂肪组织中 7、14、28、35、42 或 56 天时发生的绿色荧光蛋白阳性基质血管成分细胞的分化情况。
共植入后 14 天内荧光信号强度急剧下降，此后继续下降，56 天时信号强度为 17.3%（相对于第 1 天）。免疫荧光染色显示，一些绿色荧光蛋白阳性细胞可在第 7 天自发分化为脂肪细胞，一些植入的基质血管成分细胞可整合到新的血管中。
作者提供了令人信服的证据，证明了基质血管成分细胞与脂肪移植物共植入后发生了动态变化。结果证明了植入的基质血管成分细胞可以在脂肪移植物的缺血微环境中存活并参与脂肪生成和血管生成过程的原理。
