Ecole Normale Supérieure, Département de chimie, 24, rue Lhomond, 75005 Paris, France.
Analyst. 2013 Oct 7;138(19):5627-38. doi: 10.1039/c3an00807j.
3-Methoxy-17α-ethynylestradiol or mestranol is a prodrug for ethynylestradiol and the estrogen component of some oral contraceptive formulations. We demonstrate here that a single core multimodal probe for imaging - SCoMPI - can be efficiently grafted onto mestranol allowing its tracking in two breast cancer cell lines, MDA-MB-231 and MCF-7 fixed cells. Correlative imaging studies based on luminescence (synchrotron UV spectromicroscopy, wide field and confocal fluorescence microscopies) and vibrational (AFMIR, synchrotron FTIR spectromicroscopy, synchrotron-based multiple beam FTIR imaging, confocal Raman microspectroscopy) spectroscopies were consistent with one another and showed a Golgi apparatus distribution of the SCoMPI-mestranol conjugate in both cell lines.
3-甲氧基-17α-乙炔基雌二醇或炔雌醇是炔雌醇的前药，也是一些口服避孕药制剂的雌激素成分。我们在这里证明，一种用于成像的单一核心多模式探针 - SCoMPI - 可以有效地接枝到炔雌醇上，从而可以在两种乳腺癌细胞系 MDA-MB-231 和 MCF-7 固定细胞中对其进行追踪。基于发光（同步辐射紫外光谱显微镜、宽场和共焦荧光显微镜）和振动（AFMIR、同步辐射傅里叶变换光谱显微镜、基于同步加速器的多光束傅里叶变换成像、共焦拉曼显微光谱学）光谱学的相关成像研究相互一致，并显示 SCoMPI-炔雌醇缀合物在两种细胞系中的高尔基体分布。
