Division of Digestive Surgery, Department of Surgery, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Kamigyo-ku, Kyoto 6028566, Japan.
Anticancer Res. 2013 Aug;33(8):3185-93.
We examined the levels of long non-coding RNAs (lncRNAs) in the plasma of patients with gastric cancer to assess their clinical significance for diseases diagnosing and monitoring.
We investigated the stability of plasma lncRNAs, and then confirmed the appropriateness of the lncRNA assay with a pre-amplification method. The levels of plasma lncRNAs, H19, HOX antisense intergenic RNA (HOTAIR), and metastasis associated lung adenocarcinoma transcript-1 (MALAT1), were then analyzed in patients with gastric cancer (GC) and healthy controls.
Plasma lncRNAs exhibited minimal gradual instability only under several severe conditions. Analysis showed that samples with pre-amplification had a higher level of linearity in the reverse transcription polymerase chain reaction (RT-PCR) assay than those without pre-amplification. Plasma H19 levels were significantly higher in patients than in healthy controls. Plasma H19 levels were significantly reduced in postoperative samples.
Circulating lncRNAs can be detectable in plasma, and the detection of circulating lncRNAs may provide new complementary tumor markers for gastric cancer.
我们检测了胃癌患者血浆中的长非编码 RNA（lncRNA）水平，以评估其在疾病诊断和监测方面的临床意义。
我们研究了血浆 lncRNA 的稳定性，然后通过预扩增方法证实了 lncRNA 检测的适宜性。分析了胃癌（GC）患者和健康对照者血浆 lncRNA、H19、HOX 反义基因间 RNA（HOTAIR）和转移相关肺腺癌转录物-1（MALAT1）的水平。
lncRNA 仅在几种严重条件下表现出最小的逐渐不稳定性。分析表明，经过预扩增的样本在逆转录聚合酶链反应（RT-PCR）检测中的线性度高于未经预扩增的样本。胃癌患者血浆 H19 水平明显高于健康对照组。术后样本中血浆 H19 水平明显降低。
循环 lncRNA 可在血浆中检测到，检测循环 lncRNA 可能为胃癌提供新的补充肿瘤标志物。
