基于医学数据分析的循环长非编码 RNA、Zfpm2-As1 和 XIST 可能是胃癌诊断的潜在血浆生物标志物。
Circulating long noncoding RNA, Zfpm2-As1, and XIST based on medical data analysis are potential plasma biomarkers for gastric cancer diagnosis.
机构信息
Clinical Laboratory, The Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, China.
Clinical Laboratory, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning, China.
出版信息
Technol Health Care. 2024;32(6):4919-4928. doi: 10.3233/THC-232033.
BACKGROUND
Long noncoding RNAs (lncRNAs) participate in diseases, especially tumorigenesis, including gastric cancer (GC). Although lncRNAs in GC tissues have been extensively studied in previous research, the possible significance of circulating lncRNAs in diagnosing GC is still unknown.
OBJECTIVE
The present work investigated lncRNAs ZFPM2-AS1 and XIST with high expression in GC tissues proved as potential plasma biomarkers from 20 early GC cases, 100 GC cases, and 90 normal subjects.
METHODS
The possible correlation between ZFPM2-AS1 and XIST expression levels was analyzed with general characteristics and clinicopathological features. The performance in diagnosis was assessed according to receiver operating characteristic (ROC) analysis.
RESULTS
According to the results, XIST and ZFPM2-AS1 expression remarkably increased within GC plasma relative to normal subjects (P< 0.01); besides, lncRNA XIST expression after surgery had a tendency of downregulation compared with preoperative levels (P< 0.05). Moreover, the area under ROC curve (AUC) values were 0.62 for ZFPM2-AS1 and 0.68 for XIST, while the pooled AUC value of CA-724 and two lncRNAs was 0.751.
CONCLUSION
Circulating lncRNAs ZFPM2-AS1 and XIST can serve as the candidate plasma biomarkers used to diagnose GC.
背景
长链非编码 RNA（lncRNA）参与疾病，尤其是肿瘤发生，包括胃癌（GC）。尽管之前的研究已经广泛研究了 GC 组织中的 lncRNA，但循环 lncRNA 诊断 GC 的可能意义仍不清楚。
目的
本研究从 20 例早期 GC 病例、100 例 GC 病例和 90 例正常对照中，研究了在 GC 组织中表达较高的 lncRNA ZFPM2-AS1 和 XIST，它们被证明是潜在的血浆生物标志物。
方法
用一般特征和临床病理特征分析 ZFPM2-AS1 和 XIST 表达水平之间的可能相关性。根据接收者操作特征（ROC）分析评估诊断性能。
结果
结果表明，与正常对照组相比，GC 血浆中 XIST 和 ZFPM2-AS1 的表达明显升高（P<0.01）；此外，与术前水平相比，术后 lncRNA XIST 表达有下调的趋势（P<0.05）。此外，ZFPM2-AS1 的 ROC 曲线下面积（AUC）值为 0.62，XIST 的 AUC 值为 0.68，而 CA-724 和两个 lncRNA 的合并 AUC 值为 0.751。
结论
循环 lncRNA ZFPM2-AS1 和 XIST 可作为诊断 GC 的候选血浆生物标志物。
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