Department of Molecular Pathology, Nara Medical University, 840 Shijo-cho, Kashihara, Nara, 634-8521, Japan.
Anticancer Res. 2013 Aug;33(8):3295-300.
To establish the role of oxidative stress and v-akt murine thymoma viral oncogene homolog (AKT) activation in gastric cancer development, we examined the levels of phosphorylated AKT (pAKT), inducible nitric oxide synthase (iNOS), nitrotyrosine (NT), and human telomerase reverse transcriptase (hTERT) by enzyme-linked immunosorbent assay in 73 non-cancerous gastric mucosa and 10 gastric carcinomas. We found that the levels of pAKT were associated with the levels of iNOS, NT, and hTERT. Gastric mucosa was classified into four categories: chronic gastritis without Helicobacter pylori (CG), chronic active gastritis with H. pylori (CAG), chronic metaplastic gastritis without H. pylori (CMG), and chronic gastritis with atypia without H. pylori (CGA). We found increasing levels of pAKT, iNOS, and NT in the order of CG, CAG, CMG, and CGA. hTERT was detected only in CGA. These findings suggest that oxidative stress might be associated with AKT activation and hTERT induction and that mucosa in CGA might confer a high-risk status for gastric carcinogenesis.
为了确定氧化应激和 v-akt 鼠胸腺瘤病毒癌基因同源物 (AKT) 激活在胃癌发展中的作用，我们通过酶联免疫吸附试验检测了 73 例非癌性胃黏膜和 10 例胃癌中磷酸化 AKT (pAKT)、诱导型一氧化氮合酶 (iNOS)、硝基酪氨酸 (NT) 和人端粒酶逆转录酶 (hTERT) 的水平。我们发现 pAKT 的水平与 iNOS、NT 和 hTERT 的水平相关。胃黏膜分为 4 类：无幽门螺杆菌的慢性萎缩性胃炎 (CG)、有幽门螺杆菌的慢性活动性胃炎 (CAG)、无幽门螺杆菌的慢性化生性胃炎 (CMG) 和无幽门螺杆菌的异型增生性胃炎 (CGA)。我们发现 pAKT、iNOS 和 NT 的水平依次升高，CG、CAG、CMG 和 CGA。hTERT 仅在 CGA 中被检测到。这些发现表明，氧化应激可能与 AKT 激活和 hTERT 诱导有关，并且 CGA 中的黏膜可能赋予胃癌发生的高风险状态。
