Biomarkers. 1997;2(1):45-50. doi: 10.1080/135475097231968.
To investigate the involvement of oxidative stress in coaldust induced respiratory disorders, red blood cell and serum antioxidants in 66 coal miners were related to 5-year changes in coal workers' pneumoconiosis (CW P), chronic bronchitis, and lung function decrease (n = 40). Reduced (GSH) and oxidized (GSSG) glutathione concentrations, glutathione peroxidase (Gpx), glutathione-S-transferase (GST), superoxide dismutase (SOD), and catalase activities were measured in erythrocytes and vitamin A, vitamin E and iron were determined in serum. Changes in CWP were determined by chest radiography, chronic bronchitis was determined from a validated questionnaire and lung function decline was calculated by linear regression for a 10 year interval before blood sampling. SOD activity was increased in miners with progression of CWP (2308±156 vs 1703±155 U g(-1) Hb, p < 0.05), and GSH was reduced in those with chronic bronchitis at follow-up (3.53±0.16 vs 4.0±0.09 mmol g(-1) Hb, p < 0.01). Stepwise discriminant analysis showed that for both pneumoconiotic and non-pneumoconiotic respiratory disease in this cohort, increased enzymatic antioxidants (i.e. Gpx, Catalase, SOD) were high risk factors, while increased 'nonenzym atic' antioxidants (i.e. vitamin E, GSH) indicated reduced risk. GST activity showed discriminative power in two ways, i.e. decreased activity in those at risk for CWP, but increased in those with rapid decline in FEV1. We conclude that the multiple marker approach applied here shows the relevance of interpretation of total 'antioxidant status' versus single antioxidant measurements in health screening of individuals at risk for respiratory impairments.
为了研究氧化应激在煤尘诱导的呼吸系统疾病中的作用，我们对 66 名煤矿工人的红细胞和血清抗氧化剂进行了研究，这些工人的相关数据与为期 5 年的煤工尘肺（CWP）、慢性支气管炎和肺功能下降（n = 40）变化有关。在红细胞中测量了还原型（GSH）和氧化型（GSSG）谷胱甘肽浓度、谷胱甘肽过氧化物酶（Gpx）、谷胱甘肽-S-转移酶（GST）、超氧化物歧化酶（SOD）和过氧化氢酶活性，以及血清中的维生素 A、维生素 E 和铁。通过胸部 X 射线确定 CWP 的变化，通过验证问卷确定慢性支气管炎，通过线性回归计算采血前 10 年的肺功能下降。在 CWP 进展的矿工中，SOD 活性增加（2308±156 比 1703±155 U g(-1)Hb，p < 0.05），在随访中有慢性支气管炎的矿工中，GSH 减少（3.53±0.16 比 4.0±0.09 mmol g(-1)Hb，p < 0.01）。逐步判别分析表明，在该队列中，对于尘肺和非尘肺呼吸系统疾病，增加的酶抗氧化剂（即 Gpx、Catalase、SOD）是高风险因素，而增加的“非酶”抗氧化剂（即维生素 E、GSH）则表明风险降低。GST 活性有两种判别能力，即 CWP 高危人群的活性降低，但 FEV1 快速下降的人群活性增加。我们得出的结论是，这里应用的多标记方法表明，在对有呼吸系统损伤风险的个体进行健康筛查时，解释总“抗氧化状态”与单个抗氧化剂测量的相关性具有重要意义。
