Biomarkers. 1998;3(2):157-63. doi: 10.1080/135475098231318.
Recently there has been evidence that contaminants other than organophosphate and carbamate pesticides may inhibit the activity of the enzyme acetylcholinesterase (AChE) both under in vitro and in vivo conditions. In this study we investigated the in vitro effect of three detergents [dodecyl benzyl sulphonate (DBS), sodium dodecyl sulphate (SDS) and a mixture commonly used as domestic detergent (X)] and three metals [molybdenum, barium and chromium (VI)] on AChE activity of Mytilus galloprovincialis haemolymph. All the detergents tested significantly inhibited the activity of the enzyme. The lowest observed effect concentrations were equal to 12 5 for DBS and 50 mg l-1 for SDS and X. Among the metals, molybdenum and barium had no effect on AChE activity, whereas chromium (VI) significantly depressed the activity of the enzyme at concentrations equal to or higher than 25 mg l-1. These results suggest that the use of AChE as a specific biomarker for organophosphate and carbamate pesticides should be questioned and that the use of this enzyme as a biomarker could be extended.
最近有证据表明，除了有机磷和氨基甲酸酯类农药之外，其他污染物在体外和体内条件下都可能抑制乙酰胆碱酯酶（AChE）的活性。在这项研究中，我们研究了三种洗涤剂[十二烷基苯磺酸钠（DBS）、十二烷基硫酸钠（SDS）和一种常用的家用洗涤剂混合物（X）]和三种金属[钼、钡和铬（VI）]对贻贝血液中 AChE 活性的体外影响。所有测试的洗涤剂都显著抑制了酶的活性。最低观察到的效应浓度相当于 DBS 的 125 和 SDS 及 X 的 50mg/L。在这些金属中，钼和钡对 AChE 活性没有影响，而铬（VI）在浓度等于或高于 25mg/L 时显著抑制了酶的活性。这些结果表明，将 AChE 用作有机磷和氨基甲酸酯类农药的特异性生物标志物应该受到质疑，并且可以扩展使用这种酶作为生物标志物。
