Santos Larissa G, Oliveira Daniel C, Santos Michele S L, Neves Lia Mara G, de Gaspi Fernanda O G, Mendonça Fernanda A S, Esquisatto Marcelo A M, Santos Gláucia M T, d'Avila M A, Mei Lucia H Innocentini
Department of Materials Engineering and Bioprocess, School of Chemical Engineering, State University of Campinas-UNICAMP, Av. Albert Einstein 500, CEP 13083-852, Campinas/SP, Brazil.
J Nanosci Nanotechnol. 2013 Jul;13(7):4694-702. doi: 10.1166/jnn.2013.7194.
Biomaterials nanofibrous electrospun with biodegradable polymers have the advantage of the similarity to natural extracellular matrices, showing promising as scaffolds for application in tissue engineering. Sedum dendroideum is a phytotherapic drug that stands out for its healing properties and anti-inflammatory. This study presents the efficacy of PLA electrospun membranes used as support S. dendroideum extract releasing on excisional skin lesions of Wistar rats. The PLA porous membranes, which are nonwoven fibrous mats, were obtained by electrospinning using a conventional apparatus with a flat collector. The animals were randomly divided into nine groups: control (C), animals treated with electrospun membranes of PLA (M), animals treated with extract of S. dendroideum dissolved in saline (F), animals treated with membranes of PLA with 10% S. dendroideum (MF10), animals treated with membranes of PLA with 25% S. dendroideum (MF25). Tissue samples were taken after 2, 6 and 10 days after surgery and were subjected to structural analysis and morphology. The experimental observations showed the application of the phytotherapic incorporated in the membrane promoted a significant response regarding the number of inflammatory cells, percentage of mature collagen fibers and epithelium birrefringent in thickness excisional skin lesions in Wistar rats. It was also demonstrated that the application of the PLA membranes without the extract promoted similar responses tissues.
用可生物降解聚合物电纺的纳米纤维生物材料具有与天然细胞外基质相似的优势，有望作为组织工程中的支架应用。垂盆草是一种植物疗法药物，以其愈合特性和抗炎作用而著称。本研究展示了聚乳酸（PLA）电纺膜作为支持物用于释放垂盆草提取物对Wistar大鼠切除性皮肤损伤的疗效。聚乳酸多孔膜是无纺纤维垫，通过使用带有平板收集器的传统装置进行电纺获得。动物被随机分为九组：对照组（C）、用聚乳酸电纺膜治疗的动物（M）、用溶解在盐水中的垂盆草提取物治疗的动物（F）、用含10%垂盆草的聚乳酸膜治疗的动物（MF10）、用含25%垂盆草的聚乳酸膜治疗的动物（MF25）。在手术后2、6和10天采集组织样本，并进行结构分析和形态学观察。实验观察表明，膜中所含植物疗法药物的应用对Wistar大鼠切除性皮肤损伤中的炎症细胞数量、成熟胶原纤维百分比和上皮厚度双折射产生了显著反应。还证明了不含提取物的聚乳酸膜的应用也促进了类似的组织反应。
