Binotto Juliane Peliçari, Mendes Larissa Giorgetti, Gaspi Fernanda Oliveira de Gaspari, Esquisatto Marcelo Augusto Marreto, Andrade Thiago Antonio Moretti de, Mendonça Fernanda Aparecida Sampaio, Santos Gláucia Maria Tech
Centro Universitário, Fundação Hermínio Ometto, Araras, SP, Brazil.
Department of Materials Engineering and Bioprocess, School of Chemical Engineering, Universidade Estadual de Campinas, Brazil.
Acta Cir Bras. 2020;35(3):e202000302. doi: 10.1590/s0102-865020200030000002. Epub 2020 May 11.
To evaluate the healing potential of the electrospinning membranes of Poly (Lactic Acid) (PLA) associated with Sedum dendroideum extract in burn injuries in rats.
Seventy-five rats were submitted to burn injury on their back skin: (C) untreated; (F) with daily topical application of S. dendroideum extract; (M) with electrospinning membranes of PLA; (MF10) with electrospinning membranes of PLA with 10% S. dendroideum extract; (MF25) with electrospinning membranes of PLA with 25% S. dendroideum extract. Tissue samples were taken after 2, 6 and 14 days of the burn injury and were subjected to histomorfometric analysis of quantification of fibroblasts, collagen fibers, blood vessels, and inflammatory infiltrate Results: The histomorphometric analysis showed an increase in the number of fibroblasts, collagen fibers and blood vessels in the burns treated with membranes of PLA, associated or not with the 10% and 25% extract. The extract of S. dendroideum promoted the increase of collagen fibers.
The electrospinning PLA membrane, isolated or associated with the S. dendrodeum extract, favored the healing of burn injuries in this experimental model, with an increase of fibroblasts, collagen fibers, and blood vessels. S. dendroideum isolated only stimulated the collagenesis.
评估聚乳酸（PLA）静电纺丝膜与垂盆草提取物联合应用对大鼠烧伤创面的愈合潜力。
75只大鼠背部皮肤遭受烧伤：（C）未治疗；（F）每日局部应用垂盆草提取物；（M）使用PLA静电纺丝膜；（MF10）使用含10%垂盆草提取物的PLA静电纺丝膜；（MF25）使用含25%垂盆草提取物的PLA静电纺丝膜。在烧伤后2天、6天和14天采集组织样本，对成纤维细胞、胶原纤维、血管和炎性浸润进行组织形态计量分析。结果：组织形态计量分析显示，无论是否联合10%和25%提取物，用PLA膜治疗的烧伤创面中成纤维细胞、胶原纤维和血管数量均增加。垂盆草提取物促进了胶原纤维的增加。
在该实验模型中，单独使用或与垂盆草提取物联合使用的PLA静电纺丝膜有利于烧伤创面的愈合，表现为成纤维细胞、胶原纤维和血管数量增加。单独使用垂盆草仅刺激了胶原生成。
