Iafiscol Michele, Quirici Nadia, Foltran Ismaela, Rimondini Lia
Dipartimento di Scienze della Salute, Università del Piemonte Orientale, via Solaroli 17, 28100 Novara, Italy.
J Nanosci Nanotechnol. 2013 Jul;13(7):4720-6. doi: 10.1166/jnn.2013.7195.
Titanium and its alloys are the current materials to manufacture oral implants because of their excellent mechanical properties and biocompatibility. However the increasing needs of the patients to receive fast and reliable rehabilitation have forced materials scientists to modified the surface of the materials in order to increase the rate of osseointegration and minimize the times for healing. The presence of a reconstituted extracellular matrix (ECM), constituted of proteins and polysaccharides is a key factor for healing and regeneration of the tissues. The nano-fibrous feature of ECM improves cells proliferation and addresses their phenotype. Electrospinning technique is an efficient processing method to manufacture micro- and nano-sized fibrous structures mimicking the ECM. In this work we describe a method to obtain collagen coating made of nano-fibers onto titanium for oral implant manufacturing, using electrospinning. The obtained collagen coatings showed morphology, structure and chemical composition similar to that of ECM. Moreover the stem cells cultured onto titanium samples coated with electrospun collagen showed faster osteoblastic differentiation and more efficient deposition of mineralized matrix in comparison with those onto uncoated substrates. This effect was amplified using osteogenetic media.
钛及其合金因其优异的机械性能和生物相容性，是目前用于制造口腔植入物的材料。然而，患者对快速可靠康复的需求不断增加，迫使材料科学家对材料表面进行改性，以提高骨整合率并缩短愈合时间。由蛋白质和多糖构成的重组细胞外基质（ECM）的存在是组织愈合和再生的关键因素。ECM的纳米纤维特性可促进细胞增殖并决定其表型。静电纺丝技术是一种制造模仿ECM的微米和纳米级纤维结构的有效加工方法。在这项工作中，我们描述了一种使用静电纺丝在钛上获得由纳米纤维制成的胶原蛋白涂层以用于口腔植入物制造的方法。所获得的胶原蛋白涂层的形态、结构和化学成分与ECM相似。此外，与未涂层的钛样品相比，培养在静电纺丝胶原蛋白涂层的钛样品上的干细胞显示出更快的成骨细胞分化和更有效的矿化基质沉积。使用成骨培养基可放大这种效果。
